The Evolutionary Physiology and Ecology Laboratory occupies three rooms in Jordan Hall. One room is dedicated for meeting and shared office space, a second for project participant sampling (phlebotomy, body composition analyses, metabolic rate measures, etc.), and a third for the main wet lab. The main wet lab is equipped with its own uninterruptible power supply. A dedicated air handling system keeps the facility under monitored negative air pressure. Samples are stored in either liquid nitrogen or in a bank of locked ultra-low freezers with CO2 backup. Entry to the facility is restricted by keycard access to lab members only. Lab personnel are certified by Indiana University for working with chemical reagents and blood-borne pathogens. Secretarial and grant administrative services are available through the Department of Anthropology.

Basic equipment includes two Nuaire class II B2 biological safety cabinets, two Revco Ultima II ultralow freezers, a Nor-Lake chromatography refrigerator and enzyme freezer, a New Brunswick Galaxy 170R incubator, MVE cryogenic storage equipment, an Invitrogen Countess cell counter, an Eppendorf Mastercycler Pro thermal cycler, several Eppendorf Research Pro electric pipetters (single and multi-channel) and Mixmate shakers, a Miele G7893 glassware washer, Thermo waterbaths with Lab armor beads, a Millipore Milli-Q Integral 10 water system, a Beckman 22R microcentrifuge and a Beckman Avanti J-26XPI biosafe centrifuge system.

Specialized capital equipment includes a Bio-Tek Synergy 2 multimode plate reader, a Bio-Tek ELx405 Select microplate washer, a Luminex 200 IS flow cytometer, a Perkin Elmer Wizard 2470 gamma counter with two detectors, a Medical Graphics CCM Express indirect calorimeter and a Bodystat Quadscan 4000 bioelectrical impedance analyzer.