Our lab is investigating new sampling and emitting techniques for electrospray ionization mass spectrometry (ESI-MS), matrix-assisted laser desorption/ionization (MALDI), and other MS methods. Through use of nanopipettes, or capillaries pulled to less than 100 nm in size, as sample emitters, higher signal-to-noise ratios are observed in MS analyses when compared to commercial ESI-MS emitters. Moreover, the size scale of nanopipettes is amenable to biological sampling. Together, these contributions sum to give improved mass spectra from biologically relevant systems.
Yuill, E.M.; Sa. N.; Ray, S.J.; Hieftje, G.M.; Baker, L.A. Electrospray ionization from nanopipette emitters with tip diameters of less than 100 nanometers, Anal. Chem., 2013, accepted. (http://dx.doi.org/10.1021/ac402214g)