Malcolm E. Winkler

Professor of Biology
Director of the Biotechnology Program

Office: Jordan Hall A414

Phone: 812/856-1318

Email: mwinkler indiana.edu

Education

Ph.D., Johns Hopkins University, 1979
Helen Hay Whitney Postdoctoral Fellow, Stanford University, 1979-1982

Research

Physiology, pathogenesis, molecular genetics, stress responses, and genomics of the gram-positive human respiratory pathogenic bacterium, Streptococcus pneumoniae

Representative Publications

Winkler, M. E. and J. A. Hoch. 2008. Essentiality, Bypass, and Targeting of the YycFG (VicRK) Two-Component Regulatory System in Gram-Positive Bacteria. J. Bacteriol. published 1 February 2008, 10.1128/JB.01682-07 http://jb.asm.org/cgi/content/abstract/JB.01682-07v1?papetoc.

Ramos-Montañez S., H.-C. T. Tsui, K. J. Wayne, J. L. Morris, L. E. Peters, F. Zhang, K. M. Kazmierczak, L.-T. Sham, and M. E. Winkler. 2008. Polymorphism and Regulation of the spxB (Pyruvate Oxidase) Virulence Factor Gene by a CBS-HotDog Domain Protein (SpxR) in Serotype 2 Streptococcus pneumoniae. Mol. Microbiol. 67: 729-746.

Lanie J.A., W.-L. Ng, K. M. Kazmierczak, T. M. Andrzejewski, T. M. Davidsen, K. J. Wayne, H. Tettelin, J. I. Glass, and M. E. Winkler. 2006. Genome Sequence of Avery's Virulent Serotype 2 Strain D39 of Streptococcus pneumoniae and Comparison With That of Unencapsulated Laboratory Strain R6. J. Bacteriol. 189: 38-51.

Ng, W.-L, H-C. T. Tsui, and M. E. Winkler. 2005. Regulation of the pspA Virulence Factor and Essential pcsB Murein Biosynthetic Genes by Phosphorylated VicR (YycF) Response Regulator in Streptococcus pneumoniae. J. Bacteriol. 187 : 7444-7459.

Gilmour R., J. E. Foster, Q. Sheng, J. R. McClain, A. Rilly, P.-M. Sun, W.-L. Ng, D. Yan, T. I. Nicas, K. Henry, and M. E. Winkler. 2005. A New Class of Competitive Inhibitor of Bacterial Histidine Kinases. J. Bacteriol. 187 : 8196-8200.

Ng, W.L., K.M. Kazmierczak, and M.E. Winkler. 2004. Defective Cell Wall Synthesis in Streptococcus pneumoniae R6 Depleted for the Essential PcsB Putative Murein Hydrolase or the VicR (YycF) Response Regulator. Mol. Microbiol. 53: 1161-1175 (including cover picture for this issue).

Ng, W. L. and M.E. Winkler. 2004. Singular Structures and Operon Organizations of Essential Two Component Systems in Species of Streptococcus. Microbiology, 150: 3096-3098.

Gooding, K.B., R. Higgs, B. Hodge, E. Staffer, B. Heinz, K. McKnight, K. Phipps, M. Shapiro, M. Winkler, W.-L. Ng, and R.K. Julian. 2004. High throughput screening of library compounds against an oligonucleotide substructure of an RNA target. J Am Soc Mass Spectrom 15: 884-892.

Foster, J.E., Q. Sheng, J.R. McClain, M. Bures, T.I. Nicas, K. Henry, M.E. Winkler, and R. Gilmour. 2004. Kinetic and Mechanistic Analyses of New Classes of Inhibitors of Two-Component Signal Transduction Systems Using a Coupled Assay Containing HpkA-DrrA from Thermotoga maritima. Microbiology 150: 885-896.

Ng, W.L., G.T. Robertson, K.M. Kazmierczak, J. Zhao, R. Gilmour, and M.E. Winkler. 2003. Constitutive Expression of PcsB Suppresses the Requirement for the Essential VicR (YycF) Response Regulator in Streptococcus pneumoniae R6. Mol. Microbiol. 50: 1647-1663.