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Indiana University Bloomington

Department of Biology

Faculty & Research

Faculty Profile

Tuli Mukhopadhyay

Photo of Tuli Mukhopadhyay
Research Images
Research photo by Tuli Mukhopadhyay

Left: Cryo-EM image of purified Sindbis particles flash frozen in vitreous ice. Middle: Structure of Sindbis virus at 20 Å resolution determined by cryo-EM and 3D image reconstruction techniques. Right: Cross section of Sindbis virus at 11 Å resolution with the glycoproteins (E1 and E2), the lipid bilayer, and the capsid protein (NCP) all identified (Zhang, Mukhopadhyay et al., 2002).

Associate Professor of Biology

IU Affiliations
Biochemistry

Contact Information
By telephone: 812-856-3686/6-3633(lab)
SI 220C / SI 019 (lab)

Mukhopadhyay Lab website

Program
Microbiology
Research Area
Virology
Education

Ph.D., University of Illinois at Chicago, 1996

Postdoctoral Fellow, University of Texas, Southwestern Medical Center, 1996-1999

Postdoctoral Fellow, Purdue University, 1999-2005

Awards

IU Trustees Teaching Award, 2013

Research Description

Alphavirus Structure and Assembly

My lab is focused on examining molecular interactions required for efficient assembly and release of infectious virus particles. We work with alphaviruses which are enveloped, positive-strand RNA viruses that are transmitted to a variety of hosts by mosquitoes. Their life cycle is comprised of receptor mediated host-cell infection, viral-host membrane fusion, genome replication, virus assembly and budding. Efficient budding is fundamental for successful and continual virus infection. Alphavirus virions are icosahedral and have an external diameter of ~700-Å. Each particle consists of the viral genome, 240 copies of the capsid and spike proteins, and a host-derived lipid bilayer. Our lab is interested in identifying how the genome, proteins, and lipids assemble to gether into an infectious particle. We use a combination of genetic, biochemical, cell biological, and structural approches to study virus assembly. Specifically, we are interested in (1) identifying pro- and anti-viral factors in the host cell, (2) creating nanoparticles for both single-particle studies and virus-based biomedical applications, (3) regulation of protein folding for spike assembly, (4) role of the lipid membrane from different hosts in particle stability and infectivity, and (5) structures of assembly intermediates. For more information about each project, please visit our lab webpage.

Select Publications

Snyder, A.J., Mukherjee, S., Glass, J. K., Kearns, D. B., and Mukhopadhyay, S. (2014). The canonical twin-arginine translocase components are not required for secretion of folded GFP from B. subtilis. J. Applied and Environmental Microbiology. Appl. Environ. Microbiol., 80(10):3219-3232.  [article]

Li, Z., Dilger, J. M., Pejaver, V., Smiley, D., Arnold, R., Mooney, S., Mukhopadhyay, S., Radivojac, P., and Clemmer, D. (2014) Intrinsic Size Parameters for Palmitoylated and Carboxyamidomethylated Peptides. Inter. J. Mass Spec. 368:6-14.

Tsetsarkin, K.A., Chen, R., Yun, R., Rossi, S.L., Plante, K.S., Guerbois, M., Forrester, N., Perng, G.C., Sreekumar, E., Leal, G., Huang, J., Mukhopadhyay, S., and Weaver, S.C. (2014) Multi-peaked Aedes albopictus-adaptive landscape for chikungunya virus evolution predicts continued increases in transmissibility. Nat Commun. 16(5):4084-4097.  [article]

Sokoloski, K.J., Snyder, A.J., Liu, N.H., Hayes, C.A., Mukhopadhyay, S., and Hardy, R.W. (2013) Encapsidation of host-derived factors correlates with enhanced infectivity of Sindbis virus.  J. Virol. 87(22):12216-12226.  [article]
Cheng, F., Tsvetkova, I. B., Khuong, Y., Moore, A., Arnold, R. J., Goicochea, N. L., Dragnea, B., and Mukhopadhyay, S. (2013) The packaging of different cargo into enveloped viral nanoparticles. Mol. Pharm. 10(1):51-8.  [article]
Tsvetkova, I. B., Cheng, F., Ma, X., Moore, A. W., Howard, B., Mukhopadhyay, S., and Dragnea, B. (2013) Fusion of mApple and Venus fluorescent proteins to the SIndbis virus E2 protein leads to different cell-binding properties.  Virus Res. 177(2):138-146.  [article]
Sokoloski, K. J., Hayes, C. A., Dunn, M. P., Balke, J. L., Hardy, R. W., and Mukhopadhyay, S. (2012) Sindbis Virus Infectivity Improves During the Course of Infection in Both Mammalian and Mosquito Cells. Virus Res. 167(1):26-33.  [article]
Snyder, A. J. and Mukhopadhyay, S. (2012) The Alphavirus E3 protein functions in a clade-specific manner. J. Virol. 86(24):13609-20.  [article]
Snyder, A. J., Sokoloski, K. J., and Mukhopadhyay, S. (2012) Mutating conserved cysteines in the alphavirus E2 glycoprotein causes virus-specific assembly defects. J. Virol. 86(6):3100-11.  [article]
Cheng, F. and Mukhopadhyay, S. (2011) Generating virus-like particles with in vitro assembled cores. Virology. 413(2): 153-60.  [article]
Zlotnick, A. and Mukhopadhyay, S. (2011) Assembly, allostery, and antivirals. Trends Microbiol. 19(1): 14-23.  [article]
Parrott, M. M., Sitarski, S. A., Arnold, R. J., Picton, L.. K., Hill, R. B., and Mukhopadhyay, S. (2009) Role of Conserved Cysteines in the Alphavirus E3 Protein. J. Virol. 83(6): 2480-2490.  [article]
Goicochea, N. L, De, M., Rotello, V. M., Mukhopadhyay, S., and Dragnea, B. (2007) Core-like Particles of an Enveloped Animal Virus can Self-Assemble Efficiently on Artificial Templates. NanoLetters. 7(8):2281-90.  [article]

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