- Contact Information
- Contact Christopher Puccia by email:email@example.com
- JH A315
- Lacefield Lab website
- Department of Biology:
Genome, Cell & Developmental Biology
- B.S. Biology, 2010, Gonzaga University
- Floyd Plant and Fungal Biology Summer Fellowship, Indiana University, (2014)
NIH Training Grant in Molecular, Cellular, and Molecular Sciences (2014-2016)
Diploid budding yeast cells will undergo two meiotic divisions when exposed to starvation conditions, resulting in four haploid spores. In a process called return-to-growth, if nutrient media is provided to the cells while undergoing meiosis, they will exit meiosis and enter mitosis, provided they have not progressed past the meiotic commitment point. Upon reaching the meiotic commitment point, previously demonstrated to be during prometaphase I, cells will continue to finish meiosis even if they are subjected to nutritive growth conditions. If cells inappropriately return-to-growth after this meiotic commitment point, an increase in genome copy number can result.
The pathways that control the meiotic divisions are primarily regulated by phosphorylation. Because of this, I am interested in identifying the kinases specifically responsible for maintaining meiotic commitment. Using live fluorescent microscopy and microfluidics, nutritive media and other small molecules are flown into cells undergoing the meiotic divisions, allowing me to assess meiotic commitment in various genetic backgrounds.
- Covo, S., Puccia, C. M., Argueso, J. L., Gordenin, D. A., & Resnick, M. A. (2013). The Sister Chromatid Cohesion Pathway Suppresses Multiple Chromosome Gain and Chromosome Amplification. Genetics, genetics-113.159.202
- Zhang, H., Zeidler, A. F., Song, W., Puccia, C. M., Malc, E., Greenwell, P. W., Mieczkowski, P. A., Petes, T. D., & Argueso, J. L. (2013). Gene copy-number variation in haploid and diploid strains of the yeast Saccharomyces cerevisiae. Genetics, 193(3), 785-801.
- Shea, J., Kersten, G. J., Puccia, C. M., Stanton, A. T., Stiso, S. N., Helgeson, E. S., & Back, E. J. (2012). The use of parasites as indicators of ecosystem health as compared to insects in freshwater lakes of the Inland Northwest. Ecological Indicators, 13(1), 184-188.
- Puccia et al. (2014) Genetics, Cellular and Molecular Sciences Training Grant Symposium. “A Microfluidics Approach to Identifying Genes Involved in Meiotic Commitment.” Poster.