- Contact Information
- Contact Rachel Samson by email:email@example.com
- By telephone: 812-856-0747 (lab)
- SI 410
- Bell Lab website
- Molecular and Cellular Biochemistry
- Education and Training
- B.S. Microbiology, 2000, The Ohio State University
Career Development Fellow, 2006, Medical Research Council, Cancer Cell Unit, Cambridge, UK
Research Associate, 2007-2012, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
- Graduated Magna Cum Laude from Ohio State University, 2000
NIH Genetics, Cellular and Molecular Sciences Training Grant, 2014
Duplication of genetic material is essential for all forms of life. In several species from the archaeal domain of life, DNA replication is initiated in a sequence-specific manner at multiple sites on a single chromosome. In our studies of the archaeon Sulfolobus islandicus, we find that the three origins or replication are exclusively specified by three distinct initiation factors. Two of these factors are homologous to the eukaryotic Orc1 and Cdc6 proteins. The cycle of ATP binding and hydrolysis by at least one of the Orc1/Cdc6 homologs appears to regulate the activity of this protein at its cognate origin locus. In combination with cell cycle transcriptomic and proteomic studies we have performed, we propose a model whereby ATP hydolysis serves as a simple binary switch to control initiator activity at origins of replication. This mechanism of control ensures that all origins fire only once in a cell cycle, thus maintaining genome stability.
- Samson, R.Y., Xu, Y., Gadelha, C., Stone, T.A., Faqiri, J.N., Li, D., Qin, N., Pu, F., Liang, Y.X., She, Q., Bell, S.D. (2013) Specificity and function of archaeal DNA replication initiator proteins. Cell Rep. 3 (2): 485-496.
- Samson, R.Y., Obita, T., Hodgson, B., Shaw, M.K., Chong, P.L., Williams, R.L., Bell, S.D. (2011) Molecular and structural basis of ESCRT-III recruitment to membranes during archaeal cell division. Mol Cell. 41 (2): 186-196.
- Samson, R.Y., Obita, T., Freund, S.M., Williams, R.L., Bell, S.D. (2008) A role for the ESCRT system in cell division in archaea. Science. 322 (5908): 1710-1713.
- Recruitment of ESCRT-III during cell division, Archaeal Genetic and Molecular Mechanisms, 2011 (speaker).