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Multidimensional Separations Methods
Biomolecular mixtures containing more than a few components become increasingly
more difficult to analyze as the number of components increases. The presence
of different charge states (in the case of electrosprayed ions), sequence
isomers, and other species having overlapping mass spectral peaks makes
complete assignment of components impossible by mass spectrometry alone.
The timescale of ion mobility (milliseconds) provides unique opportunities for multidimensional separations. Nesting ion mobility separation between
condensed phase (seconds-to-minute timescales) separations and time-of-flight
mass spectrometry (microsecond timescales) is particularly desirable because
of the different modes of separation involved in these methods.
Current projects in this area include interfacing HPLC and CE separations with
our existing ion mobility/TOF apparatus and investigating the capabilities
of a FAIMS
(field asymmetric waveform ion mobility spectrometry)/ion mobility/TOF
arrangement (in collaboration with Guevremont and coworkers).
A picture of the FAIMS/ion mobility/TOF instrument is shown below.
Click here for more information on our efforts in mobility-labeled sequencing methods.
Relevant
Publications:
LC-IMS-TOF:
Srebalus Barnes, C. A.;
Hilderbrand, A. E.; Valentine, S. J.; Clemmer, D. E.
Resolving Isomeric Peptide Mixtures: A Combined HPLC/Ion Mobility-TOFMS Analysis
of a 4000-Component Combinatorial Library,
Anal. Chem. 2002, 74, 26-36.
Valentine, S. J.; Kulchania, M.; Srebalus Barnes, C. A.; Clemmer, D. E. Multidimensional separations of complex peptide mixtures: a combined high-performance liquid chromatography/ion mobility/time-of-flight mass spectrometry approach, Int. J. Mass Spectrom. 2001, 212, 97-109. A special issue celebrating R. Graham Cooks' 60th birthday.
Last modified: October 23, 2006