Biology | Molecular Biology Laboratory
L323 | 1410-1411 | Surzycki, S
Course Format: Two afternoon laboratories per week (1:00P-5:00P, MW or
Prerequisites: Course in Molecular biology or consent of instructor.
Course Description: Theory and techniques used in molecular biology
will be presented. The following techniques will be emphasized:
- Genomic DNA purification and isolation (human and bacterial)
- RNA purification and characterization
- Restriction enzyme analysis
- Southern blot analysis
- Preparation of cloning vectors and cloning DNA using electroporation
- Selection and assay of cloned and amplified fragments of "foreign" DNA
- Preparation of nonradioactive DNA probes and DNA hybridization
- Chemiluminescent detection of nucleic acids
- Preparation of random fragments sequencing libraries of human DNA
- DNA sequencing of human dsDNA clones (your own)
- Application of computers of DNA sequencing data analysis
- RFLP analysis
- Fingerprinting of human DNA (your own)
- PCR technology as applied to human genome analysis
- RAPD analysis of genomic DNA
- RT-PCR of human genes (your own)
Required Text: "Molecular Biology Laboratory", by Stefan Surzycki.
Weekly Assignments: One chapter from the manual, and viewing one VCR
"tape of the week."
Exams/Papers: Three exams and three lab reports.