Biology | Cell Biology Laboratory
L313 | 1184 | Surzycki, J

Course Format: One afternoon laboratory, one lecture and one
discussion section per week.

Prerequisites:  P: Biol L113 and L211, or Chem C342, or consent of
instructor. R: Biol L312, Chem C484.

Course Description: The course includes both techniques and theory
applied in the study of modern cell biology. It begins with more
classic experiments on cell respiration, photosynthesis and
karyotype analysis, and then introduces more contemporary cell
biology methods such as the Yeast Two-Hybrid System™ to study in
vivo protein: protein interactions affecting signal transduction
pathways, and programmed cell death using the induction of apoptosis
in human tissue culture cells. Finally there is a unit on protein
purification using a one-step protein purification system and an
expression vector. We analyze the purified protein by gel
electrophoresis using the NuPage® system, Western transfer and
immuno-detection of the purified protein.

Required Text: Cell Biology Laboratory Manual published by RL
Simonson Studios

Weekly Assignments: Flow sheet, quiz, laboratory notebook.

Exams/Papers: Two laboratory reports, a test upon the completion of
each exercise.