Biology | Microbial Physiology Laboratory
M360 | 0596 | Brun, Y
Course format: Laboratory (1:25P-4:00P, MW, JH A402) with discussions of
forthcoming work and discussions at the conclusion of labs.
Requirements: P: or concurrent - Biol M350 or consent of the instructor.
Course description: Analysis of bacterial growth, nutrition, responses to
the environment and analysis of metabolic processes. Techniques for cell
fractionation and purification of cellular components. Enzyme purification
and kinetics, and the analysis of bacterial cell proteins by
electrophoresis and column chromatography.
Required text: "Microbial Physiology & Biochemistry Laboratory" by D.
White and G. Hegeman, 1998.
Weekly assignments: Readings (including some background) in preparation for
labs and ca. six-eight hours of lab work per week.
Exams/papers: Individual or team reports on laboratory work with questions
on aspects of the lab.