Biology | Microbial Physiology Laboratory
M360 | 0564 | Brun, Y
Course format: Laboratory (1:25P-5:00P, MW, JH A402) with discussions
of forthcoming work and discussions at the conclusion of labs.
Requirements: P: or concurrent - Biol M350 or consent of the instructor.
Course description: Analysis of bacterial growth, nutrition, responses
to the environment and analysis of metabolic processes. Techniques
for cell fractionation and purification of cellular components.
Enzyme purification and kinetics, and the analysis of bacterial cell
proteins by electrophoresis and column chromatography.
Required text: “Fundamental Laboratory Approaches for Biochemistry
and Biotechnology” by Alexander J. Ninfa and David P. Ballou, 1998.
Weekly assignments: Readings (including some background) and protocol
design in preparation for labs and ca. six-eight hours of lab work per
Exams/papers: Individual or team reports on laboratory work with
questions on aspects of the lab. Quiz at the beginning of lab period.