Biology | Cell biology Lab
L313 | 29577 | Holdeman, R


The course includes both techniques and theory applied in the study
of modern cell biology. We begin with more classic experiments on
cell respiration and photosynthesis and then onto karyotype
analysis. We then introduce more contemporary cell biology methods
such as the Yeast Two-Hybrid Systemô to study in vivo protein:
protein interactions affecting signal transduction pathways and
programmed cell death using the induction of apoptosis in human
tissue culture cells. We end with a unit on protein purification
using a one-step protein purification system and an expression
vector. We analyze the purified protein by gel electrophoresis (SDS-
PAGE), and Western transfer and immuno-detection of the purified
protein.