From: PO3::"quaternary@morgan.ucs.mun.ca" 1-FEB-1995 02:48:03.20 To: sweets@ucs.indiana.edu CC: Subj: Re: Exotic pollen tablets supplier of exotic pollen tablets for >absolute pollen method. Either Lycopodium or Eucalyptus >would be suitable. Can anyone give me a supplier. Contact Dr. B. Berglund, Lund, Sweden at e-mail: bjorn.berglund@geol.lu.se His lab has been making tablets for years now. Good luck ----------------------- DR. SUZANNE A. G. LEROY Associate director, PAGES CPO, Baerenplatz 2, CH-3011 Berne, Switzerland Ph.: 41-31-3123133; fax: 3123168; e-mail: leroy@pageigbp.unibe.ch and pages@ubeclu.unibe.ch From: PO2::"quaternary@morgan.ucs.mun.ca" 1-FEB-1995 10:36:57.32 To: sweets@ucs.indiana.edu CC: Subj: RE: Exotic pollen tablets > I am looking for a supplier of exotic pollen tablets for > absolute pollen method. Either Lycopodium or Eucalyptus > would be suitable. Can anyone give me a supplier. > > Bob Mott > A "new" batch of Lycopodium "tracers" is available for calculating native pollen concentrations. batch 124951 X=12,542 for one tablet contact Thomas.Persson@geol.lu.se Owen Owen K. Davis, Ph.D. Phone 602 621 7953 Professor of Geosciences FAX 602 621 2672 University of Arizona palynolo@ccit.arizona.edu Tucson, AZ 85721 From: PO3::"quaternary@morgan.ucs.mun.ca" 1-FEB-1995 11:59:16.75 To: sweets@ucs.indiana.edu CC: Subj: Re: Exotic pollen tablets Bob; The latest issue of AASP newsletter has an article (p. 17) on Lycopodium tablets. Batch 124961 is now available at 150 SEK/bottle (500 tablets) plus shipping. 1 SEK ~= C$0.18. They cannot take personal cheques, but do take POs. Orders should be sent to: Bjo"rn Berglund and Thomas Persson Department of Quaternary Geology Lund University Tornva"gen 13 S-223 63 Lund Sweden FAX: 46-46-104830. Bob: if you need a few to tide you over until your order comes in, let me know. Cheers, | Ian D. Campbell | Canadian Forest Service | 5320-122 St. Edmonton, AB | Canada T6H 3S5 | Tel: +1-403-435-7300 Fax: +1-403-435-7359 | e-mail: icampbell@nofc.forestry.ca *****************************************************************************************************************From: PO3::"QUATERNARY@MORGAN.UCS.MUN.CA" "Canadian Research in Quaternary Science" 16-OCT-1995 12:23:47.63 To: Multiple recipients of list QUATERNARY CC: Subj: Re: Pollen Tracers Dear Bob, We use polystyrene microspheres to spike our pollen samples. Last fall, we started ordering them from DuPont NEN (New England Nuclear Research Products). The spheres we ordered from NEN were the non-radioactive 15 micron microspheres, catalog # NEM-002 - a 500 mg lot in a 50ml suspension of 20% of Dextran with Tween to prevent clumping. The shipment we recieved contained about 1.8995 x 10 -8 beads (they will give you the approximate # of beads if you ask them for it when you place your order, which is kind of nice). NEN can be reached by calling 1-800-551-2121. Their customer service address is: DuPont NEN Research Products Customer Service 549 Albany Street Boston, MA 02118 When making your less-concentrated suspensions, you can use dextran, but I'd advise a 7-10% concentration of Dextran rather than a 20% concentration, as the latter is awfully sticky and can cause clumping, even with Triton X or Tween added. Also, I was told by one of the NEN lab scientists to sonicate the concentrated suspension you are sent for 15 minutes, then swirl it, and sonicate it once again before making up the other suspensions. I follow this same procedure immediately before I add the spheres to my samples, swirling in between samples. Now - having given you this info, I am compelled to let you know that these babies are pretty expensive. They cost about 320 dollars. But, the reason we use them is that they are the right density - they can be added at the very beginning of processing. They won't float out with HF treatment. The only possible drawback with this product is that the spheres are black, so if you have a lot of charcoal or pyrite in you sample, they might be obscured. I have not had this problem. Previously, we had been using microspheres frome Duke Scientific, which were cheaper (and light-colored), but because of their lesser density, had to be added after HF treatment. However, if bucks are a factor, Duke's phone # is 1-800-334-3883, and their fax is 415-962-0718. They are in Palo Alto, California. I think we paid about 100 dollars for the last batch we order from them. Either way, the amount that you recieve is adequate, once diluted down the the right concentration, to make numerous spikes. It's not something I use up rapidly. And then there are Eucalyptus grains. I have not used them, but they may be an even better answer for you. Hope I have helped in some way! Pietra Gardetto Mueller Illinois State Museum Research and Collections Center 1011 E. Ash St. Springfield, IL 62703 Mueller@Museum.state.il.us Pietra Gardetto Mueller From Reed.Scherer@NATGEOG.UU.SETue Apr 29 14:58:32 1997 Date: Fri, 18 Oct 1996 08:51:05 +0200 From: Reed Scherer Reply to: Research in Quaternary Science To: Multiple recipients of list QUATERNARY Subject: MORE ON "diatom tablets" At 12:43 PM 10/17/96 -0500, you wrote: >At 03:19 PM 10/17/96 +1300, you wrote: >>This is a general query. >> >>Is there any equivalent in diatom studies to the addition of known pollen >>aliquots or tablets to pollen samples ? >> >>If there is, does anybody know of a supplier of diatom/glass bead tablets ? >> >>Thanks >> >>Jamie Shulmeister > > >I have a preprint of a paper by Rick Battarbee and M.J. Kneen entitled "The >Use of Electronically Counted Microspheres in Absolute Diatom Analysis" with >"L&O in press" written on the top of it along with the date 1982. I assume >this did actually appear in L&O (Rick are you out there?). THIS REF IS: LIMN. OCE. 27:184-188 > >The following reference is also useful: > >Ogden, J.G., III. 1986. An alternative to exotic spore or pollen addition in >quantitative microfossil studies. Canadian Journal of Earth Sciences 23:102-106. > >This reference also discusses the use of plastic microspheres. More >recently, we've obtained microspheres similar to the ones Ogden described. >For more information, go to the Paleolimnology Web page >http://www.indiana.edu/~diatom/paleo.html and click on the "Sources of >exotic pollen and other markers" link near the bottom of the page (or go >directly to http://www.indiana.edu/~diatom/exopol.ann) and see the comments >by Pietra Mueller. ALSO SEE: KALAND & STABELL, 1981. METHODS FOR ABSOLUTE DIATOM FREQUENCY ANALYSIS AND COMBINED DIATOM AND POLLEN ANALYSIS IN SEDIMENTS. NORDIC J. BOT. 1:697-700. MOST RECENT PAPER DISCUSSING THE SUBJECT IS: SCHERER, 1994. A NEW METHOD FOR THE DETERMINATION OF ABSOLUTE ABUNDANCE OF DIATOMS AND OTHER SILT-SIZED SEDIMENTARY PARTICLES. J. PALEOLIMN. 12:171-179. REED SCHERER ============================================================================ Reed Scherer Institute of Earth Sciences - Quaternary Geology Uppsala University Norbyvagen 18B S-752 36 Uppsala Sweden 46 18 182575 (office) 46 18 555920 (fax) 46 171 445039 (residence) Reed.Scherer@natgeog.uu.se From grimm@MUSEUM.STATE.IL.USTue Apr 29 14:59:16 1997 Date: Mon, 21 Oct 1996 11:51:14 -0500 From: "Eric C. Grimm" Reply to: Research in Quaternary Science To: Multiple recipients of list QUATERNARY Subject: Re: microspheres More on the diatom-microsphere question from Rick Battarbee: >Dear Eric, > >Hi, yes it was published in L & O 1982, 27, 184-188. The other paper that >describes quite a bit of diatom methodology is: > >Battarbee, R.W. 1986 Diatom analysis. In Berglund, B.E. (ed.) Handbook of >Holocene Palaeoecology and Palaeohydrology. John Wiley & Sons, Chichester, >pp. 527-570. > >We use divinylbenzene microspheres with a mean diameter of 5.7 micrometres, >and can provide calibrated suspensions. It's used like a laboratory re- >agent - dilute the stock suspension and add a known weight to the diatom >prep. If anyone needs a supply they can contact Viv Jones on vjones@ >ucl.ac.uk. > >All the best > >Rick > ******************************************************************* Dr. Eric C. Grimm Office: 217-785-4846 Illinois State Museum Database: 217-524-0493 Research and Collections Center Fax: 217-785-2857 1011 East Ash Street E-mail: grimm@museum.state.il.us Springfield, IL 62703 USA 39d,46m,48s N, 89d,38m,34s W *******************************************************************