Giedroc Group

Publications

Metal Sensor Proteins and Metalloregulation of Transcription

A Cu(I)-sensing ArsR family metal sensor protein with a relaxed metal selectivity profile

Liu, T., Chen, X., Ma, Z., Shokes, J., Hemmingsen, L, Scott, R. A., Giedroc, D. P. (2008)
Biochemistry 47, 10564-10575.
View: PDF | PubMed | Supplementary Material

Copper sensing by Drosophila metal-responsive transcription factor-1 requires a Cu(I)4-S6 cluster.

Chen, X., Hua, H., Balamurugan, K., Kong, X., Wang, L., George, G. N., Georgiev, O., Schaffner, W. and Giedroc, D. P. (2008)
Nucl. Acids Res., 36, 3128-3138.
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Multiple metal-binding domains enhance the Zn(II) selectivity of the divalent metal ion transporter AztA.

Liu, T., Reyes, H., Li, C., Li, C., Scott, R. A., & Giedroc, D. P. (2007)
Biochemistry 46, 11057-11068.
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Metal Sensor Proteins: Nature's metalloregulated allosteric switches.

Giedroc, D. P. and Arunkumar, A. I. (2007)
Dalton Trans., 3107-3120 (invited Perspective).
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Resonance assignments of the metal sensor CzrA in the apo-, Zn2- and DNA-bound (42 kDa) states.

Arunkumar, A. I., Pennella, M. A., Kong, X., and Giedroc, D. P. (2007)
Biomol. NMR Assign 1, 99-101.
View: PDF | SpringerLink

CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator.

Liu, T., Ramesh, A., Ma, Z, Ward, S. K., Zhang, L., George, G. N., Talaat, A. M., Sacchettini, J. C., & Giedroc, D. P. (2007)
Nature Chem. Biol. 3, 60-68.
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Individual Metal Ligands Play Distinct Functional Roles in the Zinc Sensor Staphylococcus aureus CzrA.

Pennella, M.A., Arunkumar, A.I., & Giedroc, D. P. (2006)
J. Mol. Biol. 356, 1124-1136.
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Structural Insights into Homo- and Heterotropic Allosteric Coupling in the Zinc Sensor S. aureus CzrA from Covalently Fused Dimers.

Lee, S., Arunkumar, A.I., Chen, X., & Giedroc, D. P. (2006)
J. Am. Chem. Soc. 128, 1937-1947.
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Kinetics of Metal Binding by the Toxic Metal-Sensing Transcriptional Repressor S. aureus pI258 CadC.

Busenlehner, L.S., and Giedroc, D. P. (2006)
J. Inorg. Biochem 100, 1024-1034.
View: PubMed

Structural Determinants of Metal Selectivity in Prokaryotic Metal-Responsive Transcriptional Regulators.

Pennella, M. A., & Giedroc, D. P. (2005)
Biometals 18, 413-428 (invited review).
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A Zn(II)/Pb(II)/Cd(II)-Inducible Operon from Anabaena is Regulated by AztR, an alpha3N ArsR/SmtB-family Metalloregulator.

Liu, T., Golden, J. W., & Giedroc, D. P. (2005)
Biochemistry 44, 8673-8683.
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Structural and Functional Characterization of M. tuberculosis CmtR, a Pb(II)/Cd(II)-sensing SmtB/ArsR Metalloregulatory Repressor.

Wang, Y., & Hemmingsen, L., & Giedroc, D. P. (2005)
Biochemistry 44, 8976-8988.
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A Novel Cyanobacterial SmtB/ArsR Family Metalloregulator Regulates a CPx-ATPase and a Metallothionein in Response to both Cu(I)/Ag(I) and Zn(II)/Cd(II).

Liu, T., Nakashima, S., Hirose, K., Shibasaka, M., Katsuhara, M., Ezaki, B., Giedroc, D. P., & Kasamo, K. (2004)
J. Biol. Chem. 279, 17810-17818.
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A Novel Cysteine Cluster in Human MTF-1 is Required for Heavy Metal-induced Transcriptional Activation in vivo.

Chen, X., Zhang, B., Harmon, P. M., Schaffner, W., Peterson, D. O., & Giedroc, D. P. (2004)
J. Biol. Chem. 279, 4515-4522.
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Ratiometric Pulsed Alkylation-Mass Spectrometry as a Probe of Thiolate Reactivity in Different Metalloderivatives of S. aureus pI258 CadC

Apuy, J. A., Busenlehner, L. S., Russell, D. H., & Giedroc, D. P. (2004)
Biochemistry 43, 3824-3834.
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A Metal-Ligand mediated Intersubunit Allosteric Switch in Related SmtB/ArsR Zinc Sensor Proteins.

Eicken, C., Pennella, M. A., Chen, X., Koshlap, K., VanZile, M. L., Sacchettini, J. C., & Giedroc, D. P. (2003)
J. Mol. Biol. 333, 683-695.
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The SmtB/ArsR Family of Metalloregulatory Transcriptional Repressors: Structural Insights into Prokaryotic Metal Resistance.

Busenlehner, L. S., Pennella, M. A., & Giedroc, D. P. (2003)
FEMS Microbiol. Rev. 27, 131-144 (invited review).
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Structural Elements of Metal Selectivity in Metal Sensor Proteins.

Pennella, M. A., Shokes, J. E., Cosper, N. J., Scott, R. A., & Giedroc, D. P. (2003)
Proc. Natl. Acad. Sci. USA 100, 3713-3718.
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A Nickel-Cobalt Sensing ArsR-SmtB Family Repressor: Contributions of the Cytosol and Effector Binding Sites to Metal Selectivity.

Cavet, J. S., Meng, W., Pennella, M. A., Appelhoff, R. J., Giedroc, D. P., & Robinson, N. J. (2002)
J. Biol. Chem. 277, 38441-38448.
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Allosteric Negative Regulation of smt O/P Binding of the Zinc Sensor, SmtB, by Metal Ions: A Coupled Equilibrium Analysis.

VanZile, M.L., Chen, X., & Giedroc, D.P. (2002)
Biochemistry 41, 9776-9786.
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Structural and Functional Characterization of a Metalloregulatory Bi(III) Site in S. aureus pI258 CadC.

Busenlehner, L.S., Apuy, J.L., & Giedroc, D.P. (2002)
J. Biol. Inorg. Chem. 7, 551-559.
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Elucidation of Primary (alpha3N) and Vestigial (alpha5) Heavy Metal Binding Sites in S. aureus pI258 CadC: Evolutionary Implications for Metal Ion Selectivity of ArsR/SmtB Metal Sensor Proteins.

Busenlehner, L.S., Weng, T.-C., Penner-Hahn, J.E. & Giedroc, D.P. (2002)
J. Mol. Biol. 319, 685-701.
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Structural Characterization of Distinct alpha3N and alpha5 Metal Sites in the Cyanobacterial Zinc Sensor SmtB.

VanZile, M.L., Chen, X., & Giedroc, D.P. (2002)
Biochemistry 41, 9765-9775.
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Spectroscopic Properties of the Metalloregulatory Cd(II) and Pb(II) Sites of S. aureus pI258 CadC.

Busenlehner, L.S., Cosper, N.J., Scott, R.A., Rosen, B.P., Wong, M.D. & Giedroc, D.P. (2001)
Biochemistry 40, 4426-4436.
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Conformational Heterogeneity in the C-Terminal Zinc Fingers of Human MTF-1: An NMR and Zinc Binding Study.

Giedroc, D.P., X. Chen, Pennella, M., & LiWang, A. (2001)
J. Biol. Chem. 276, 42322-42332.
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Ratiometric Pulsed-Alkylation Mass Spectrometry of the Cysteine Pairs in Individual Zinc Fingers of MRE-Binding Transcription Factor-1 (MTF-1) as a Probe of Zinc Chelate Stability.

Apuy, J.L., Chen, X., Baldwin, T.O., Russell, D.H., & Giedroc, D.P. (2001)
Biochemistry 40, 15164-15175.
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Metal-Response Element-Binding Transcription Factor-1 (MTF-1): Structure, Function and Regulation.

Giedroc, D.P., Chen, X., & Apuy, J. (2001)
Antiox. Redox Signal. 3, 577-596 (invited review).
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The Zinc Metalloregulatory Protein, Synechococcus PCC7942 SmtB, Binds a Single Zinc Ion per Monomer with High Affinity in a Tetrahedral Coordination Geometry.

VanZile, M.L., Cosper, N., Scott, R.A. & Giedroc, D.P. (2000)
Biochemistry 39, 11818-11829.
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MRE-Binding Transcription Factor-1: Weak Zinc-Binding Finger Domains 5 and 6 Modulate the Structure, Affinity and Specificity of the Metal-Response Element Complex.

Chen, X., Chu, M., & Giedroc, D.P. (1999)
Biochemistry 38, 12915-12925.
View: PubMed

Structural and Functional Heterogeneity Among the Zinc-Fingers of Human MRE-Binding Transcription Factor-1 (MTF-1).

Chen, X., Agarwal, A., & Giedroc, D.P. (1998)
Biochemistry 37, 11152-11161.
View: PubMed