Giedroc Group
The Biophysical Chemistry of Infectious Disease
Bioinorganic Chemistry • Biophysical Chemistry • Bioanalytical Chemistry

Our reasearch interests fall under a common umbrella we term the biophysical chemistry of infectious disease. Work is ongoing in three separate projects: 1) transition metal homeostasis (metallostasis) in bacterial pathogens; 2) hydrogen sulfide (H2S) sensing and reactive sulfur species (RSS) in the major nosocomial pathogens, Staphylococcus aureus (MRSA) and Enterococcus facaelis; and 3) the replication of vertebrate coronaviruses, including the causative agent of Middle East Respiratory Syndrome (MERS). Prof. Giedroc seeks a molecular-level understanding of macromolecular structure, dynamics and regulation, and uses the tools of biophysical chemistry, bioinorganic chemistry, proteomic profiling and NMR structure determination.

Sulfide sensing and Reactive Sulfur Species in Bacterial Pathogens

Sulfide Sensing and Reactive Sulfur Species (RSS) in Bacteria In this project, we are developing new concepts of bacterial hydrogen sulfide (H2S) homeostasis, and seeking an understanding of the chemistry and physiological adaptation of H2S misregulation in important microbial pathogens.  In 2014, we discovered a paralog of a copper-sensing operon repressor CsoR in Staphylococcus aureus that… View Article

Metallostasis in Bacterial Pathogens

Metallostasis: Transition Metal Homeostasis in Bacterial Pathogens In this project, our goal is to understand the molecular mechanisms of how cells regulate the intracellular bioavailability of essential transition metal ions, notably Cu, Mn and Zn among others. This process, termed metal homeostasis and resistance, represents an important battleground in human host-bacterial pathogen interactions since these… View Article

Coronavirus Replication: RNA Structure and Protein-RNA Interactions

Coronavirus Replication In this project, initiated as a collaborative project, we are using the tools of biophysical chemistry SHAPE and NMR spectroscopy to understand the structure and biological function of the very “tips” of the coronavirus genome, the 5′ and 3′ untranslated regions (UTRs) that direct the replication, subgenomic RNA (sgRNA) transcription, and propagation of… View Article

Past Projects

Collaborative Projects

Cell Division and Peptidoglycan Remodeling in Streptococcus pneumoniae

Publications Structure of the large extracellular loop of FtsX and its interaction with the essential peptidoglycan hydrolase PcsB in Streptococcus pneumoniae Rued, B. E., Alcorlo, M., Edmonds, K. A., Martínez-Caballero, S., Straume, D., Fu, Y., Bruce, K. E., Wu, H., Håvarstein, L. S., Hermoso, J. A., Winkler, M. E., & Giedroc, D. P. (2018) BioRxiv (doi: 10.1101/432344)… View Article