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Indiana University Bloomington
Flow Cytometry Core Facility
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Welcome to the IUB Flow Cytometry Core Facility

The IU Bloomington Flow Cytometry Core Facility (FCCF) offers flow cytometry analysis and cell sorting services. It houses the FACS Aria II, a state-of-the-art sorter/analyzer capable of analyzing fourteen colors and seventeen parameters, the COPAS Select, a Drosophila embryo and large particle sorter, the LSRII, a thirteen color, sixteen parameter analyzer with HTS option, the FACSCalibur, a four-color analyzer, as well as Z2 Coulter counter technology used for determining cell concentration and size distribution. The facility also houses the AmpliGrid system (discontinued) for single-cell PCR. Services include technician assisted sorting, as well as training for analyzers, PCR and Coulter instruments. Help with experimental design is also available. The facility maintains a workstation with flow cytometry data analysis software, and also has a copy of this software for use outside of the facility. For more information and for any questions, please contact the facility.


Fall 2015 - The FCCF is offering the Z620 Flow Cytometry course again this fall. It is open to all graduate students. First 8 weeks.

April 13-14 - the Facility hosts Justin Meyers for a flow cytometry Lunch and Learn seminar and instrument demonstration on Monday April 13. Researchers may sign up to run fixed or BSL1 samples on Tuesday April 14. Please RSVP for lunch, demo and sample analysis by clicking here: survey

BioTechniques article about acknowledgment of core facilities in publications: http://www.biotechniques.com/multimedia/archive/00248/BTN_A_000114233_O_248450a.pdf

Holiday closing - the FCCF closes 5pm Friday December 19 and reopens Monday January 5 - HAPPY HOLIDAYS!

October 2014 - The facility has renewed its membership in the Indiana CTSI - https://www.indianactsi.org/

Fall 2014 - the FCCF is offering an Introduction to Flow Cytometry Z620 course for the second time (8-week course)

Indiana CTSI funding - Fall 2014 core pilot funding for use of CTSI designated core facilities is due Monday September 29 by 4pm: https://www.indianactsi.org/grantinfo/grant/172

July 2014 - July 7: Foot pedals were successfully installed at the flow facility sink: please use foot pedals for hand washing purposes, July 11: an extra faucet was added to the sink for decontaminated waste disposal; this faucet should NOT be used for hand washing, July 14: the annual IU-B Flow Core User Satisfaction survey will be emailed to the facility listserv (survey deadline is August 15, 11:59pm) - flow facility survey can be found at: IU-B Flow Core Users Survey 2014

Cyto 2014 - meeting updates: check the Facebook page for information http://www.facebook.com/pages/IUB-FCCF/231178580343589 (no Facebook account needed!)

May 29, 2014 - The Calibur workstation was upgraded from OS9 to OSX. All files/templates still on the OS9 workstation were transferred.

May 2014 - Two FlowJo dongles are now available for check out and are version X. The facility workstation was upgraded to Windows 7 and runs FlowJo vX

April 9, 2014 IU Bloomington Newsroom article - "IU Bloomington faculty awarded awarded Indiana Clinical and Translational Sciences grants" (click on title to read article)

>> The IUB FCCF is a CTSI designated core - this means that researchers may apply for funds that can be used to perform research with facility equipment. More information about funding opportunities can be found at https://www.indianactsi.org/ - go to "Grants and Funding"

>> The FCCF is on Facebook - find and like us at http://www.facebook.com/pages/IUB-FCCF/231178580343589 - you do not need to be a member of Facebook to view this (just click on "close" in the bottom righthand corner of the box that pops up)


Oversight Committee

John Foley, Ph.D. (Med Sci) - Director
Kris Klueg, Ph.D. (CGB) - Associate Director
Christiane Hassel, M.S. (Biology) - Manager
Christina Dann, Ph.D (Chemistry) - Chair
Pranav Danthi, Ph.D. (Biology)
Ke Hu, Ph.D. (Biology)
Roger Innes, Ph.D. (Biology)
Thom Kaufman, Ph.D. (Biology)
Ken Nephew, Ph.D (Med Sci)