Indiana University Bloomington
Flow Cytometry Core Facility

Welcome to the IUB Flow Cytometry Core Facility

The IU Bloomington Flow Cytometry Core Facility (FCCF) offers flow cytometry analysis and cell sorting services. It houses the FACS Aria II, a state-of-the-art sorter/analyzer capable of analyzing fourteen colors and seventeen parameters, the COPAS Select, a Drosophila embryo and large particle sorter, the LSRII, a thirteen color, sixteen parameter analyzer with HTS option, the FACSCalibur, a four-color analyzer, as well as Z2 Coulter counter technology used for determining cell concentration and size distribution. The facility also houses the AmpliGrid system (discontinued) for single-cell PCR. Services include technician assisted sorting, as well as training for analyzers, PCR and Coulter instruments. Help with experimental design is also available. The facility maintains a workstation with flow cytometry data analysis software, and also has a copy of this software for use outside of the facility. For more information and for any questions, please contact the facility.


July 2018: The facility has added an FCS Express site license! If you are interested in registering for a site license ($234/year) for unlimited use of FCS Express from any computer, please contact Christiane Hassel. More information to come!

July 4-6, 2018: The facility will be closed for the 4th of July holiday

March 5, 2018: FCS Express 6 seminar; to download a demo go to:

The Flow Facility will be closed February 12-16: Facility manager out for MACSQuant training

The Flow Facility will be closed for the holidays: December 20-27, 2017, and January 1, 2018

Annual Flow Facility user satisfaction survey is available - if you did not receive a link to the survey and would like to complete it, please contact the facility manager

Fall 2017 - The FCCF is offering the Z620 Flow Cytometry course again this fall. It is open to all graduate students. First 8 weeks.

July 17 - August 11, 2017 - The facility will be offering FREE trials of the new MACSQuant VYB flow cytometer - more information about the instrument can be found in these documents: [Mercury Specs] [Mercury Trial, Rates, Q&A]

July 3-4, 2017 - The facility will be closed for the July 4th holiday

June 23, 2017 - The "Mercury" is already featured on two IU Twitter feeds:,

June 22, 2017 - The MACSQuant VYB "Mercury" mobile cytometer is here. More details about when the instrument will be available for use will be coming soon

May 16-30, 2017 - Facility closed

April 12, 2017 - Facility closed after 2pm

April 7, 2017 - Facility closed all day

March 13-24, 2017 - Exhaust fan and air handler outage affects the flow facility

February 23-24, 2017 - MACSQuant here for instrument demo

January 17 - February 22, 2017: The flow facility will be testing a NovoCyte flow cytometer. This analyzer is a 3-laser system with 405nm, 488nm, and 561nm lasers. If you would like to test the instrument while it is here, please contact the facility. More information at

>> BioTechniques article about acknowledgment of core facilities in publications:

>> The IUB FCCF is a CTSI designated core - this means that researchers may apply for funds that can be used to perform research with facility equipment. More information about funding opportunities can be found at - go to "Grants and Funding"

>> We're on Facebook - find and like us at - you do not need to be a member of Facebook to view (just click on "close" in the bottom righthand corner if a box pops up)


Oversight Committee

John Foley, Ph.D. (Med Sci) - Director
Kris Klueg, Ph.D. (DGRC) - Associate Director
Christiane Hassel, M.S. (Biology) - Manager
Pranav Danthi, Ph.D. (Biology)
Ke Hu, Ph.D. (Biology)
Roger Innes, Ph.D. (Biology) - Chair
Thom Kaufman, Ph.D. (Biology)
Ken Nephew, Ph.D (Med Sci)