Indiana University Bloomington
Flow Cytometry Core Facility

Welcome to the IUB Flow Cytometry Core Facility

The IU Bloomington Flow Cytometry Core Facility (FCCF) offers flow cytometry analysis and cell sorting services. It houses the FACS Aria II, a state-of-the-art sorter/analyzer capable of analyzing fourteen colors and seventeen parameters, the COPAS Select, a Drosophila embryo and large particle sorter, the LSRII, a thirteen color, sixteen parameter analyzer with HTS option, the FACSCalibur, a four-color analyzer, as well as Z2 Coulter counter technology used for determining cell concentration and size distribution. The facility also houses the AmpliGrid system (discontinued) for single-cell PCR. Services include technician assisted sorting, as well as training for analyzers, PCR and Coulter instruments. Help with experimental design is also available. The facility maintains a workstation with flow cytometry data analysis software, and also has a copy of this software for use outside of the facility. For more information and for any questions, please contact the facility.

Announcements

May 16-30, 2017 - Facility closed

April 12, 2017 - Facility closed after 2pm

April 7, 2017 - Facility closed all day

March 13-24, 2017 - Exhaust fan and air handler outage affects the flow facility

February 23-24, 2017 - MACSQuant here for instrument demo

January 17 - February 22, 2017: The flow facility will be testing a NovoCyte flow cytometer. This analyzer is a 3-laser system with 405nm, 488nm, and 561nm lasers. If you would like to test the instrument while it is here, please contact the facility. More information at https://aceabio.com/products/novocyte/

December 2016 Holiday closings (Christmas, New Year) - The Facility closes at 5pm Monday Dec. 20 and reopens Wednesday Dec. 28 AND closes 5pm Thursday Dec. 29 and reopens Tues. Jan. 3 - HAPPY HOLIDAYS!

November 2016 Holiday closing (Thanksgiving) - The Facility closes at 5pm Tuesday Nov. 22 and reopens Monday Nov. 28

Annual Flow Facility user satisfaction survey is available - if you did not receive a link to the survey and would like to complete it, please contact the facility manager

Fall 2016 - The FCCF is offering the Z620 Flow Cytometry course again this fall. It is open to all graduate students. First 8 weeks.

Meeting/vacation closing 2016- the facility will be closed July 25-29

>> BioTechniques article about acknowledgment of core facilities in publications: http://www.biotechniques.com/multimedia/archive/00248/BTN_A_000114233_O_248450a.pdf

>> The IUB FCCF is a CTSI designated core - this means that researchers may apply for funds that can be used to perform research with facility equipment. More information about funding opportunities can be found at https://www.indianactsi.org/ - go to "Grants and Funding"

>> We're on Facebook - find and like us at http://www.facebook.com/pages/IUB-FCCF/231178580343589 - you do not need to be a member of Facebook to view (just click on "close" in the bottom righthand corner if a box pops up)

 

Oversight Committee

John Foley, Ph.D. (Med Sci) - Director
Kris Klueg, Ph.D. (CGB) - Associate Director, Chair
Christiane Hassel, M.S. (Biology) - Manager
Pranav Danthi, Ph.D. (Biology)
Ke Hu, Ph.D. (Biology)
Roger Innes, Ph.D. (Biology)
Thom Kaufman, Ph.D. (Biology)
Ken Nephew, Ph.D (Med Sci)