Indiana University Bloomington
Flow Cytometry Core Facility
Protocols & Reagents

Protocols

The following PDF contains useful information about the facility and sample preparation:

Please fill out and submit the following forms when submitting a new project or using the Aria II (Athena):

These protocols have been used by researchers at IU Bloomington:

Useful guides:

Additional assays and sorting performed by researchers at IU Bloomington:

Other assays available:
  • BrdU incorporation
  • Cell proliferation assays
  • Intracellular Cytokine detection
  • Multiparameter Immunofluorescence analysis and cell sorting
  • Signal Transduction
  • and much more!
Other resources for cell preparation and staining for flow cytometry

Reagents

Flow Cytometry Products Available in the Biology Stockroom - prices subject to change

Product Packaging Location Cost Instrument
Falcon Tubes w/ cap
12x75mm (5ml)
Ref# 352054
125/package
Sterile
Stockroom $18.37 package Aria II
Calibur
LSRII
Falcon Tubes w/ cap
12x75mm (5ml)
Individually wrapped
Sterile
Stockroom $0.23 ea. Aria II ONLY
Falcon Tubes w/ cap
17x100mm (15ml)
Individually wrapped
Sterile
Stockroom $0.16 ea. Aria II ONLY
BD Falcon Cell strainers - 40um
Ref# 352340
Individually wrapped
Sterile
Stockroom $1.16 ea. Aria II
Calibur
LSRII
Sysmex (previously Partec) Filter - 30um
Individually wrapped
Sterile
Stockroom $1.57 ea. Aria II
Calibur
LSRII
Sysmex (previously Partec) Filter - 50um
Individually wrapped
Sterile
Stockroom $1.65 ea. Aria II
Calibur
LSRII
Plastic container – 27qt w/ Lid N/A Stockroom $9.49 ea. For carrying samples to FCCF
Disposable Lab coat (various sizes) N/A Stockroom

M - $3.32 ea.
L - $4.49 ea.
XL - $3.50 ea.

All
The FCCF supplies cloth lab coats

Safety Glasses Box Stockroom $6.39 ea. All
Gloves Box Stockroom Various types, sizes, prices available All
The FCCF supplies Xceed nitrile gloves