Gay Rights Info, highly useful privately maintained site with Congressional and state legislator voting records, extensive info on state laws and policies, court decisions, employer info, and other unique or hard-to-find resources
Rainbow Query, "the largest, most complete GLBT search on the internet"

About.com's gay/lesbian issues guide
Amazon Online, resources for lesbians and bi women (not affiliated with the book site)
Blackstripe, "the internet's leading resource for news, information and culture affecting LGBT people of African descent"
Gay.com, large commercial site featuring arts, health, finance, travel, news, chat, personals, etc.
Gaywired.com -- entertainment, news, events, shopping, etc.
Indiana University GLBT Student Support Services Office subject or geographic guide to national and worldwide GLBT resources
Lesbian.org
LesbiaNation, entertainment, sports, business, lifestyle, more
PlanetOut -- major commercial site with something for everyone, somewhat stronger news/politics content than Gay.com
Pridelinks.com, index and search engine that claims 8,000+ links, including local, state, and international sites
Queer Resources Directory:  Home | Subject tree | Links elsewhere (tons of info, though regrettably not being very well maintained or updated)
Queerlinks.com -- M.J. O'Neill's huge site with links to just about everything queer imaginable
Suite101: Lesbian issues -- a selection of articles, links, discussions
The Other Queer Page, over 1,400 sorted links