Indiana Section of the Society for Applied SpectroscopyIndiana Section of the Society for Applied Spectroscopy
1999-2000 Seminar Abstracts
FORMATION, STRUCTURAL CHARACTERIZATION, AND REACTIVITY OF ORGANOSULFUR MONOLAYERS ON GOLD: AN INFRARED SPECTROSCOPY AND FORCE MICROSCOPY INVESTIGATION
Jeremy Kenseth, Sze-Shun Wong, Hajime Takano, Marc Porter
Organosulfur monolayers have been employed extensively as models for a wide range of interfacial processes. This presentation describes two such applications: the use of thiol-derived monolayers chemisorbed on gold as coupling agents for a novel detection format for immunoassays, and the use of the same general architecture as substrates for the extension of surface compositional mapping techniques. Both applications couple the compositional and architectural insights provided by infrared reflection spectroscopy (IRS) and the topographical and friction force imaging capabilities of atomic force microscopy (AFM). IRS is used in both cases as a probe of the formation, structural arrangements, and chemical modification of the different adlayers. The AFM is used in the former application to detect the changes in topography that arise from the specific binding of an antibody to a patterned array of immobilized antigens, and in the latter application to detect frictional differences that arise from differences, for example, in the orientation of the terminal groups of the adlayer. Details of the surface patterning processes are also described.
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