Welcome to the Macromolecular Crystallography Facility (MCF) at Indiana University, Bloomington.
The MCF provides all the equipment necessary to facilitate research in all aspects of structural biology at IUB. New instrumentation allows high throughput macromolecular crystallography by the use of advanced robotics. The Phoenix DT and Gryphon LCP drop setter robots comercialized by Art Robbins Intruments can set up crystalization trails with high accuracy and minimizing the total amount of purified sample used during the screening. The Minstrel HT UV (Rigaku) is a fully automated high-throughput ultraviolet and visible crystal imaging and protein crystal monitoring system, which is coupled to two Gallery™ HT incubator storage systems. Using the CrystalTrak sofware in situ or online the users can control, optimize, visualize and document automaticaly all the crystallization screening process. In addition, the Alchemist DT (Rigaku) also connected to CrystalTrak software, allows automatic liquid handling for protein crystallization optimization, eliminating the tedious process of solution preparation and dispensing by hand. Steroscopes coupled to high definition camaras and supplies for crystal manipulation are also provided by the facility. Lastly, computational resources facilitate structure determination and refinement with the latest programs available. The facility encourages research endeavors in structural biology and provides a central resource for IUB users as well as surrounding Academic Institutions as well as Commercial Entities. If you have any questions or need more information, please feel free to contact the Facility Manager, Giovanni Gonzalez-Gutierrez.