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Light Microscopy Imaging Center

Correlative Light and Electron Microscopy (CLEM) Workshop

May 14, 2014

Registration       Program         Sponsors

Correlative light and electron microscopy (CLEM) combines the advantages of the two separate microscopy platforms to image the same sample at vastly different resolutions. Light microscopy can provide images of organisms, whole cells and cell organelles. The use of fluorescently labeled proteins allows for the localization of specific proteins and also allows us to watch the dynamic reorganization of labeled proteins in real time, but even the best super-resolution images are limited to resolutions no better than 30 - 100 nanometers (i.e., larger than individual proteins and many macromolecular assemblies). On the other hand, electron microscopy can produce images at much higher resolution (sub-nanometer, in many cases), but only on relatively small and static regions of a organelle, cell or organism. In addition, there are rather limited ways to tag molecules of interest for visualization using EM. The developing field of CLEM examines exactly the same specimen at the different resolution scales provided by light and electron microscopy in a way that aligns features visible by light microscopy with the ultrastructure provided by electron microscopy. This combination provides complementary and often unique information that cannot be obtained when looking at specimens that are only superficially alike and that may not have even been prepared under similar conditions. IU-Bloomington's Light Microscopy Imaging Center (LMIC) and Electron Microscopy Center (EMC) have all the tools needed for CLEM. This workshop will discuss how to use these tools to take a correlative approach to various scientific questions.

 

Registration

Registration is FREE and open to all. Please email Jim Powers to indicate your interest or if you have any questions.

Program

Jordan Hall, Room 009

9:30 - 10:00am      Registration (coffee).

10:00 - Noon          Seminar Session 1

  • David Morgan - IUB
    • Introduction
  • Chris Gilpin - Purdue
    • Tools and techniques
  • Short Research Talks - Examples of a CLEM approach
    • Vamseedhar Rayaprolu- IUB
    • Roger Innes - IUB

Noon - 1:30pm         Lunch

1:30 - 3:00pm          Seminar Session 2

  • Kent McDonald - UC-Berkley
    • High Pressure Freezing
  • Derron Bishop - IUSOM/Ball State
    • Deep tissue CLEM

3:00 - 5:00pm

  • Informal discussions with speakers
  • Tours of microscopy facilities

 

 

Sponsors

This workshop is possible due to support from the following organizations. Thank You!

Indiana University: