Microscopes
BD Pathway 855 High-Content Bioimager | BD Pathway 435 High-Content Bioimager | Leica Scanning Confocal |Spinning Disk Confocal | Nikon C1 Scanning Confocal
| Deltavision | Nikon E800 | Zeiss Axioplan | Veritas Microdissection System
BD Pathway 855 High-Content Bioimager
Purchased September 2008, the 855 is a versatile system for high-content confocal imaging of live or fixed samples. It can handle specimen formats from single slides to multiwell plates. It can automatically image multiple sites per slide or well and every well of a multiwall plate. The 855 includes powerful imaging software to perform a broad range of fluorescence-based kinetic and endpoint biological assays.
Features:
- Confocal fluorescence, widefield fluorescence, brightfield.
- X-cite lamps to provide full spectrum illumination (340nm-700nm).
- Hamamatsu Orca ER cooled CCD camera.
- Handles slides to multiwell plates.
- Laser and image based autofocus.
- Environmental control.
- Liquid handling.
- Robotics to handle loading/changing of multiwell plates.
- BD Pathway imaging software and BD AttoVision Software for image processing and analysis.
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BD Pathway 435 High-Content Bioimager
Also acquired September 2008, the 435 is similar to the 855, but specialized for imaging fixed specimens. It can handle specimen formats from single slides to multiwell plates. It can automatically image multiple sites per slide or well and every well of a multiwall plate. Both machines include the same powerful imaging software to perform a broad range of fluorescence-based kinetic and endpoint biological assays.
Features:
- Confocal fluorescence, widefield fluorescence, brightfield.
- X-cite lamp to provide full spectrum illumination (340nm-700nm).
- Hamamatsu Orca ER cooled CCD camera.
- Handles slides to multiwell plates.
- Laser and image based autofocus.
- BD Pathway imaging software and BD AttoVision Software for image processing and analysis.
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Leica SP5 Scanning Confocal
A new microscope (6/2008), the SP5 is a scanning confocal with all of the bells and whistles. This system has 4 sensitive detectors enabling the simultaneous imaging of up to 4 different wavelengths. It has 3 lasers providing 7 excitation wavelengths and a prism spectrometer to control emission wavelengths to make this a very versatile system compatible with most combinations of fluorophores.
Features:
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Leica DMI 6000 CS inverted microscope platform
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7 lines of laser illumination (458,476,488,496,514,561 and 633nm) can excite from CFP to CY-5 fluorophores.
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Can simultaneously image fluorescence and brightfield or DIC
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FRAP or FRET fully supported with rapid kinetic analysis
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Controlled by Leica LAS software
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This system is optimized for live-cell imaging. The sensitive Cascade-II EMCCD camera in conjunction with the Yokogawa CSU-10 spinning disk confocal head enables images to be captured under low light conditions with excellent temporal resolution. The reduced photobleacing/toxicity of this system allows imaging over longer time periods than our other systems.
Features:
- Nikon TE2000U Inverted Microscope platform
- FRAP/Photoactivation using the Mosaic System (Photonics Inc.)
- Lasers for Green or Red fluorophores (ie. GFP. FITC, RFP, TRITC)
- Can also collect bright-field and DIC images
Temperature control available
- Controlled by Metamorph imaging software
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Nikon C1 Scanning Confocal
New in February 2009, this system is built on the Nikon Eclipse Ti inverted microscope and includes the Perfect Focus system. This system is built to maintain focus throughout long-term live imaging experiments. It has three lasers that provide four excitation wavelengths.
Features:
- Nikon Eclipse Ti inverted microscope platform.
- The Nikon Perfect Focus system
- Four laser lines(440, 488, 514 and 543nm) to excite many of the popular fluorophores including CFP, GFP, YFP and RFP
- Simultaneous or sequential multichannel imaging.
- Controlled by EZC1 software
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The Applied Precision DeltaVision Restoration Microscopy System is optimized for deconvolution microscopy. Deconvolution is a software technique that mathematically de-blurs images. This system features a very nice motorized stage for precise control of X, Y, and Z plane movements. Multiple sites on a slide can be marked and revisited.
Features:
- Nikon Eclipse TE200 inverted microscope platform
- Photometrics CH350-CCD camera
- Hg Lamp illumination with filters for UV/DAPI, Green, Red and Far-red (CY-5) fluorophores.
- Can also collect bright-field and DIC images
- Controlled by SoftWorx imaging software
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The Nikon E800 is a very nice general purpose research microscope. It is equipped for brightfield, DIC and epifluorescence imaging. The microscope has 4X to 100X objectives so a wide range of samples can be accommodated. Epifluorescence imaging on this scope works best with thin, flat specimens.
Features:
- The Nikon Eclipse E800 is an upright microscope platform
- Hamamatsu Orca-ER CCD camera
- Hg Lamp illumination with filters for UV/DAPI, Green, Red, Far-red (CY-5) fluorophores. and Chlorophyll.
- Motorized focus control for Z-stack images
- Controlled by Metamorph imaging software
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The Axioplan is a good general purpose research microscope. It is equipped for brightfield, phase-contrast, and epifluorescence imaging. Images are captured using a Princeton Instruments RTE/CCD camera controlled by the basic Zeiss imaging software. This software, while basic, is easy to learn and easy to use.
Features:
- The Zeiss Axioplan is an upright microscope platform
- Princeton Instruments RTE/CCD-1300-Y/HS camera
- Hg Lamp illumination with filters for UV/DAPI, Green and Red fluorophores
- Easy to use, a great option for basic imaging or just to look around
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Veritas Microdissection System
The Veritas is a fully automated microscope system designed to dissect specific cells, organelles, or tissues from your specimen for further analysis. It can do both laser-cutting for a large number of cells and laser capture for single cells or organelles.
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