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Indiana University Bloomington

Christina DannChristina Dann

Associate Scientist, Chemistry

Office: Myers 216B

Phone: 812/856-7577

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Education

Postdoctoral Fellow, UT Southwestern Medical Center, 2002-2008
Ph.D., Johns Hopkins University, 2001
B.S., College of William and Mary, 1996

Research

The life-long production of specialized adult cells depends upon the maintenance of a pool of progenitor cells called stem cells.   We study spermatogonial stem cells, the progenitors of sperm.  These stem cells are unlike other adult stem cells in two interesting ways.  First, they are the source for the gametes, the only cells that carry the genetic information to the next generation.  Second, they exhibit a high degree of developmental plasticity, having the capacity to become spontaneously reprogrammed to pluripotent stem cells that could be used in therapy for a variety of diseases.   Our goals are to determine mechanisms that control spermatogonial stem cell fate while also developing technology  to facilitate cell and gene therapy using cultured mouse and human spermatogonial stem cells as model systems.

Representative Publications

Suzuki, H., Dann, C.T., Rajkovic, A. (2013) Generation of a germ cell-specific mouse transgenic CHERRY reporter, Sohlh1-mCherry-Flag.  Genesis. 51, (pp 50-58).

Heim, C., Fanslow, D., Dann, C.T. (2012) Development of quantitative microscopy-based assays for evaluating dynamics of  living cultures of mouse spermatogonial stem/progenitor cells. Biology of Reproduction. 87, (pp 90, 1-11).

Dann, C.T. (2012) Transgenic modification of spermatogonial stem cells using lentiviral vectors. In Aston, K. I. and Carrell, D. T. (Eds.), Methods in Molecular Biology:  Spermatogenesis and spermiogenesis, methods and protocols.  927, (pp 503-18).

Song, H, Dann, C.T., McCarrey, J., Meistrich, M., Cornwall, G., Wilkinson, M. F. (2012)
Dynamic expression pattern and subcellular localization of the RHOX10 homeobox transcription factor during early germ cell development. Reproduction. 143, (pp 611-24).

Minniear, K., Heim, C., Dann, C.T. (2011) Imatinib mesylate has deleterious effects on differentiated spermatogonia while sparing spermatogonial stem cell self renewal. Reproductive Toxicology. In press – (vol. 31).

Richardson T.E., Chapman K.M., Dann C.T., Hammer R.E., Hamra F.K. (2009) Sterile testis complementation with spermatogonial lines restores fertility to DAZL-deficient rats and maximizes donor germline transmission.  PLoS One.  4, (e6308).

Dann, C.T., Alvarado, A., Molyneux, L., Denard, B.S., Garbers, D.L., Porteus, M.H. (2008) Spermatogonial Stem Cell Function Requires OCT4, a Factor Down-regulated During Retinoic Acid Induced Differentiation in vitro. Stem Cells. 26, (2928-37).

Dann, C.T.  and Garbers. D.L. (2008) Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes. In Hou, S. X. and Singh S. R. (Eds.), Methods in Molecular Biology:  Germline stem cells  (pp 193-209). Totowa, NJ:  Humana Press.

Dann, C.T.  (2007) New Technology for an Old Favorite:  Lentiviral Transgenesis and RNAi in Rats.  Transgenic Research. 16, 571-580.

Dann, C.T., Alvarado, A., Hammer, R., Garbers. D. (2006) Heritable and Stable Gene Knockdown in Rats.   PNAS. 103, 11246 -11251. [PMC1544073]

Tenenhaus, C., Subramaniam, K., Dunn, M., Seydoux, G. (2001) PIE-1 is a Bifunctional Protein that Regulates Maternal and Zygotic Gene Expression in the Embryonic Germline of C. elegans. Genes and Development . 15, 1031-1040. [PMC312670]

Tenenhaus, C., Schubert, C., Seydoux, G. (1998) Genetic Requirements for PIE-1 Localization and Inhibition of Gene Expression in the Embryonic Germ Lineage of C. elegans.  Developmental Biology. 200, 212-224.