Peter C Hollenhorst
Assistant Professor, Medical Science
Office: Jordan Hall 208
B.S. 1997 St. Norbert College
Ph.D. 2002 University of Wisconsin
American Cancer Society Postdoctoral Fellow,
Huntsman Cancer Institute, University of Utah
Our lab studies the mechanisms used by sequence specific transcription factors to interact with the genome and regulate gene expression. The interpretation of genomic sequence by transcription factors allows the proper execution of gene expression programs critical for development and tissue homeostasis. Because cancer arises when genes are improperly expressed, the mechanisms controlling gene expression are also central to the problem of carcinogenesis. Our research focuses on understanding mechanisms underlying specific and redundant genomic targeting within a group of 27 human transcription factors called the ETS family. Chromosomal rearrangements that alter the expression or function of ETS transcription factors occur in a number of different cancer types including approximately one-half of all prostate cancers. We use techniques ranging from molecular biology and biochemistry to genomics and bioinformatics to understand how ETS transcription factors interact with the human genome to drive normal cell functions, and how this process goes awry in cancer.
Hollenhorst, P. (2012). RAS/ERK pathway transcriptional regulation through ETS/AP-1 binding sites. Small GTPases 1(3).
Hollenhorst, P.C., Ferris, M.W., Hull, M.A., Chae, H., Kim, S., and B.J. Graves. (2011). Oncogenic ETS proteins mimic activated RAS/MAPK signaling in prostate cells. Genes & Development 25(20): 2147-57.
Hollenhorst, P.C., Paul, L., Ferris, M.W., and B.J. Graves. (2011). The ETS gene ETV4 is required for anchorage-independent growth and a cell proliferation gene expression program in PC3 prostate cells. Genes and Cancer 1: 1044-1052.
Hollenhorst, P.C., McIntosh, L.P., and B.J. Graves. (2011). Genomic and biochemical insights into the specificity of ETS transcription factors. Annual Review of Biochemistry 80: 437-71.
Oler, A.J., Alla, R.K., Roberts, D.N., Wong, A., Hollenhorst, P.C., Chandler, K.J., Cassiday, P.A., Nelson, C.A., Hagedorn, C.H., Graves, B.J., and B.R. Cairns. (2010). Human RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors. Nature Structural and Molecular Biology 17(5): 620-628.
Clement, N.L., Snell, Q., Clement, M.J., Hollenhorst, P.C., Purwar, J., Graves, B.J., Cairns, B.R., and W.E. Johnson. (2010). The GNUMAP algorithm: unbiased probabilistic mapping of oligonucleotides from next-generation sequencing. Bioinformatics 26(1): 38-45.
Hollenhorst, P.C., Chandler, K.J., Poulsen, R.L., Johnson, W.E., Speck, N.A., and B.J. Graves. (2009). DNA specificity determinants associate with distinct transcription factor functions. PLoS Genetics 5(12): e1000778.
Gangwal, K., Sankar, S., Hollenhorst, P.C., Kinsey, M., Haroldsen, S.C., Shah, A.A., Boucher, K.M., Watkins, W.S., Jorde, L.B., Graves, B.J., and S.L. Lessnick. (2008). Microsatellites as EWS/FLI response elements in Ewing’s sarcoma. Proc Nat Acad Sci USA 105(29): 10149-10154
Hollenhorst, P.C., Shah, A.A., Hopkins, C., and B.J. Graves. (2007). Genome-wide analyses reveal properties of redundant and specific promoter occupancy within the ETS gene family. Genes & Development 21(15): 1882-1894.
Hollenhorst, P.C., Jones, D.A., and B.J. Graves. (2004). Expression profiles frame the promoter specificity dilemma of the ETS family of transcription factors. Nucleic Acids Research 32(18): 5693-5702.
Hollenhorst, P.C., Pietz, G., and C.A. Fox. (2001). Mechanisms controlling differential promoter-occupancy by the yeast forkhead proteins Fkh1p and Fkh2p: implications for regulating the cell cycle and differentiation. Genes & Development 15(18): 2445-2456.
Hollenhorst, P.C., Bose, M.E., Mielke, M.R., Muller U., and C.A. Fox. (2000). Forkhead genes in transcriptional silencing, cell morphology, and the cell cycle: Overlapping and distinct roles for FKH1 and FKH2 in Saccharomyces cerevisiae. Genetics 154(4): 1533-1548.