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Indiana University Bloomington

Malcolm WinklerMalcolm E. Winkler

Professor of Biology, Microbiology Section Associate Chair

Office: Jordan Hall A414

Phone: 812/856-1318

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Ph.D., Johns Hopkins University, 1979
Helen Hay Whitney Postdoctoral Fellow, Stanford University, 1979-1982
Infectious Diseases Research Advisor, Eli Lilly and Company, 1999-2003


Genomics and Bioinformatics, Microbial Cell Biology and Environmental Responses, and Microbial Interactions and Pathogenesis

Representative Publications

Fu, Y., K. E. Bruce, B. E. Rued, M. E. Winkler, and D. P. Giedroc. (2015). 1H, 13C, 15N Resonance Assignments of the Extracellular Loop 1 Domain (ECL1) of Streptococcus pneumoniae D39 FtsX, an Essential Cell Division Protein. Biomolec NMR Assign, in press.

Boersma, M. J., E. Kuru, J. T. Rittichier, M. S. VanNieuwenhze, Y,V. Brun, and M. E. Winkler. (2015). Minimal Peptidoglycan Turnover in Wild-Type and PG Hydrolase and Cell Division Mutants of Streptococcus pneumoniae D39 Growing Planktonically and In Host-Relevant Biofilms. J. Bacteriol, in press.

Kocaoglu O, Tsui HC, Winkler ME, Carlson EE. (2015). Profiling of β-Lactam Selectivity for Penicillin-Binding Proteins in Streptcoccus pneumoniae D39. Antimicrob Agents Chemother. 59:3548-3555.

Hoover SE, Perez AJ, Tsui HC, Sinha D, Smiley DL, DiMarchi RD, Winkler ME, Lazazzera BA.(2015). A new quorum sensing system (TprA/PhrA) for Streptococcus pneumoniae D39 that regulates a lantibiotic biosynthesis gene cluster. Molecular Microbiology, in press, published on-line Apr 13. doi: 10.1111/mmi.13029. [Epub ahead of print]. PMID: 25869931 (PMC in process).
“Subject of MicroCommentary: Monnet V, Gardan R. (2015). Quorum-sensing regulators in Gram-positive bacteria: “cherchez le peptide.” Molecular Microbiology, in press, published on-line May 19. doi: 10.1111/mmi 13060 [Epub ahead of print]. PMID: 25988215.”

Tsui H.-C. T., M. J. Boersma, S. A. Vella, O. Kocaoglu, E. Kuru E, J. K. Peceny, E. E. Carlson, M. S. VanNieuwenhze, Y. V. Brun, S. L. Shaw, and M. E. Winkler. (2014). Pbp2x Localizes Separately from Pbp2b and Other Peptidoglycan Synthesis Proteins During Later Stages of Cell Division of Streptococcus pneumoniae D39. Molec Microbiol 94:21-40. (PMC4209751).
Cover photo of volume 94, number 1 (October) of Molecular Microbiology. MicroCommentary: Cadby I.T. and A. L. Lovering. 2014. Molecular Surveillance of the Subtle Septum: Discovering a New Mode of Peptidoglycan Synthesis in Streptococcus. Molec Microbiol 94: 1-4.  

Yue F, H.-C. T. Tsui, K. E. Bruce, L.-T. Sham, K. A. Higgins, J. P. Lisher, K. M. Kazmierczak, M. J. Maroney, C. E. Dann III, M. E. Winkler, and D. P. Giedroc. (2013). A New Structural Paradigm in Copper Resistance in Streptococcus pneumoniae. Nature Chem Biol9: 177-83. (PMC3578076).

Sham, L. T., K. R.  Jensen, K. E. Bruce, and M. E. Winkler. (2013). Involvement of FtsE ATPase and FtsX Extracellular Loops 1 and 2 in FtsEX:PcsB Complex Function in Cell Division of Streptococcus pneumoniae D39. MBio 4: pii: e00431-13. doi: 10.1128/mBio.00431-13. (PMC3735124).

Land, A.D., H.-C. T. Tsui, O. Kocaoglu, S. Vella, S. L. Shaw, S. K. Keen, L.-T. Sham, E. E. Carlson, and M. E. Winkler. (2013). Requirement of Essential Pbp2x and GpsB for Septal Ring Closure in Streptococcus pneumoniae D39. Molec Microbiol 90: 939-55.(PMC4120849).
Cover photo of volume 90, number 5 (December) of Molecular Microbiology.

Kocaoglu, O., R. A. Calvo, L.T. Sham, L. M. Cozy, S. Francis, B. R. Lanning, M. E. Winkler, D. B. Kearns, and E. E. Carlson. (2012). Selective Penicillin-Binding Protein Imaging Probes Reveal Substructure in Bacterial Cell Division. ACS Chem Biol 7: 1746-1753. (PMC3663142). Highlighted by the Faculty of 1000.

Sham, L.-T,, H.-C. T. Tsui, A. D. Land, S. M. Barendt, and M. E. Winkler. (2012). Recent Advances in Pneumococcal Peptidoglycan Biosynthesis Suggest New Vaccine and Antimicrobial Targets. Curr Opin Microbiol. 15:194-203. (PMC3322672).

Wayne, K. J., S. Li, K. M. Kazmierczak, H.-C. T. Tsui, and M. E. Winkler. (2012). Involvement of WalK (VicK) Phosphatase Activity in Setting WalR (VicR) Response Regulator Phosphorylation Level and Limiting Crosstalk in Streptococcus pneumoniae D39 Cells. Molec Microbiol 86:645-60. (PMC3638944).

Tsui, H.-C. T., S. K. Keen, L.-T. Sham, K. J. Wayne, and M. E. Winkler. (2011). Dynamic Distribution of the SecA and SecY Translocase Subunits and Septal Localization of the HtrA Surface Chaperon/Protease During Cell Division of Streptococcus pneumoniae D39. mBio 2(5). pii: e00202-11. doi: 10.1128/mBio.00202-11.

Jacobsen, F. E., K. M. Kazmierczak, J. P. Lisher,M. E. Winkler, and D. P. Giedroc. (2011). Interplay between Manganese and Zinc Homeostasis in the Human Pathogen Streptococcus pneumoniae. Metallomics 3: 38-41. [cover article].

Barendt, S. M., L.-T. Sham, and M. E. Winkler. (2011). Characterization of Mutants Deficient in the L,D-Carboxypeptidase (DacB) and WalRK-Spn (VicRK) Regulon Involved in Peptidoglycan Maturation of Streptococcus pneumoniae Serotype 2 Strain D39. J. Bacteriol. 193: 2290-2300.

Land, A. D., and M. E. Winkler (2011). Requirement for Pneumococcal MreC and MreD Is Relieved by Inactivation of the Gene Encoding PBP1a. J. Bacteriol. 193: 4166-4179.

Hsueh, Y.-H., L. M. Cozy, L.-T. Sham, R. A. Calvo, A. D. Gutu, M. E. Winkler, and D. B. Kearns. (2011). DegU-Phosphate Activates Expression of the Anti-Sigma Factor FlgM in Bacillus subtilis. Molec. Microbiol. 81: 1092-1108.

Nichols, R. J., Y. J. Choo, S. Sen, P. Beltrao, M. Zietek, R. Chaba, S. Lee, K. M. Kazmierczak, K. J. Lee, A. Wong, M. Shales, S. Lovett, M. E. Winkler, N. J. Krogan, A. Typas, and C. A. Gross. (2011). Phenotypic Landscape of a Bacterial Cell. Cell 144:1-14.

Major textbook: Wilson, B. A., A. A. Salyers, D. D. Whitt, and M. E. Winkler. (2011). Bacterial Pathogenesis - A Molecular Approach. Third Edition. ASM Press. Washington, D. C.

Sham, L.-T., S. M. Barendt, K. E. Kopecky, and M. E. Winkler. (2011). Essential PcsB Putative Peptidoglycan Hydrolase Interacts with the Essential FtsXSpn Cell Division Protein in Streptococcus pneumoniae D39. Proc. National Acad. of Sci., U. S. A. 108: 1061-2069.

Biller, S. J., K. J. Wayne, M. E. Winkler, and W. F. Burkholder. (2011). The Putative Hydrolase YycJ (WalJ) Affects the Coordination of Cell Division with DNA Replication in Bacillus subtilis and May Play a Conserved Role in Cell Wall Metabolism. J. Bacteriol. 193:896-908.

Wayne, K. J., L.-T. Sham, H.-C. T. Tsui, A. D. Gutu, S. M. Barendt, S. K. Keen, and M. E. Winkler. (2010). Localization and Cellular Amounts of the WalRKJSpn (VicRKX) Two-Component Regulatory System Proteins in Serotype 2 Streptococcus pneumoniae. J. Bacteriol. 192: 4388-4394. .

Ramos-Montañez, S., K. M. Kazmierczak, K. L. Hentchel, and M. E. Winkler. (2010). Instability of ackA (Acetate Kinase) Mutations and Their Effects on Acetyl Phosphate (AcP) and ATP Amounts in Streptococcus pneumoniae D39. J. Bacteriol. 192: 6390-6400.

Reyes-Caballero, H, A. J. Guerra, F. E. Jacobsen, K. M. Kazmierczak, D. Cowart, U. M. Kumar-Koppolu, R. A. Scott, M. E. Winkler, and D. P. Giedroc. (2010). The Metalloregulatory Zinc Site in Streptococcus pneumoniae AdcR, A Zinc-Activated MarR Family Repressor. J. Mol. Biol. 403:197-216.

Gutu, A. D., K. J. Wayne, L-T. Sham, and M. E. Winkler. (2010). Kinetic Characterization of the WalRKSpn (VicRK) Two-Component System of Streptococcus pneumoniae: Dependence of WalKSpn (VicK) Phosphatase Activity on Its PAS Domain. J. Bacteriol. 192: 2346-2358..

Tsui, H.-C. T., D. Mukherjee, V. A. Ray, L.-T. Sham, A. L. Feig, and M. E. Winkler. (2010). Identification and Characterization of Non-Coding Small RNAs in Streptococcus pneumoniae Serotype 2 Strain D39. J. Bacteriol 192:264-279.

Barendt, S. M., A. D. Land, L.-T. Sham, W.-L. Ng, H.-C. T. Tsui, R. J. Arnold, and M. E. Winkler. (2009).Capsule Influences the Cell Shape and Chain Length of pcsB Mutants in Serotype 2 Streptococcus pneumoniae. J. Bacteriol 191: 3024-3040. [cover article].

Kazmierczak, K. M., K. J. Wayne, A. Rechtsteiner, and M. E. Winkler. (2009). Roles of relSpn in the Stringent Response, Global Regulation, and Virulence of Serotype 2 Streptococcus pneumoniae. Molec Micro 72: 590-611.

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