Professor, Molecular and Cellular Biochemistry Department
Office: Simon Hall 220A
BA, Biology, University of Virginia
PhD, Biology, Purdue University
Our broad goal is to examine the structure and assembly of protein oligomers, determining structures of products, reactants and intermediates, and correlating them with solution studies. A major focus of the lab is to understand the biophysics of virus capsid assembly. The capsid of a spherical of virus is assembled from multiples of 60 protein subunits, arranged with icosahedral symmetry. All projects in the lab involve multimeric viral proteins and enzymes.
Selzer, L, Katen, SP, Zlotnick, A (2014) Structural Changes at the HBV Core Protein Intra-dimer Interface Modulate Capsid Assembly and Stability. Biochemistry, e-published Aug 7.
Wang, J C-Y, Nickens, DG, Lenz, TB, Loeb, DD, Zlotnick, A (2014) Encapsidated hepatitis B virus reverse transcriptase is poised on an ordered RNA lattice. PNAS, epub July 17, 2014 PMCID in progress.
Wang,J C-Y, Zlotnick, A, Mecinović, J (2014) Transmission electron microscopy enables the reconstruction of the catenane and ring forms of CS2 hydrolase. Chem Comm, e-published July 18, 2014. PMCID in progress.
Pierson, EE, Kiefer, DZ, Selzer, L, Lee, LS, Contino, NC, Wang, JC-Y, Zlotnick, A*, Jarrold, MF* (2014) Detection of Late Intermediates in Virus Capsid Assembly by Charge Detection Mass Spectrometry. JACS 136, 3536-41. PMC3985884.
Li, L., Chirapu, SR, Finn, Zlotnick, A (2013). Phase diagrams map the properties of anti-viral agents directed against Hepatitis B virus core assembly. Antimicrobial Agents and Chemother 57, 1505-8. PMC3591873.
Zlotnick, A, Tan, Z, Selzer, L (2013) One protein, at least three structures, and many functions. Structure 21, 6-8. PMC3545281.
Tan, Z., Maguire, M. Loeb, D.D., Zlotnick, A. (2013) Genetically altering the thermodynamics and kinetics of Hepatitis B Virus capsid assembly has profound effects on virus replication in cell culture. J Virol 87-3208-16. PMC3592155
Highlighted as a J Virol spotlight.
Zlotnick, A, Porterfield, JZ, Wang, JC-Y (2013) To build a virus on nucleic acid substrates. Biophysical Journal 104, 1595-1604. PMC3617413.
Katen, SP, Tan, Z, Chirapu, SR, Finn, MG, Zlotnick, A (2013) The structure of HBV is distorted by phenylpropenamide assembly effectors. Structure 21, 1406-16. PMC3756818.
Kler, S, Wang, J C-Y, Dhason, M, Oppenheim, A, Zlotnick, A (2013) Scaffold properties are a key determinant of the size and shape of self-assembled virus-derived particles. ACS Chemical Biology 8, 2753-61. PMC3870028.
Drayman, N, Glick, Y, Ben-nun-Shaul, O, Zer, H, Zlotnick, A, GerberZer, D, Schueler-Furman, O, Oppenheim, A (2013) Pathogen-host recognition achieved by structural mimicry of native ligands via convergent evolution. Cell Host and Microbe 14, 63-73. News and Views topic, (2013). Nature 501, 38-9.
Li, H, Porterfield, JZ, van der Schoot, PPAM, Zlotnick, A, Dragnea, B (2013). Hepatitis Virus Capsid Polymorphs Respond Differently to Changes in Encapsulated Cargo Size. ACS Nano 7, 8447-54. PMCID in progress.
Kler, S, Asor, R, Li, C, Ginsberg, A, Harries, D, Oppenheim, A*, Zlotnick, A*, Raviv, U* (2012) RNA-induced assembly of SV40-derived nanoparticles is very fast and with no detectable intermediates. JACS 134, 8823-30. PMC3365646.
Zlotnick, A., Suhanovsky, MM, and Teschke, CM (2012) The energetic contributions of scaffolding protein and capsid protein to the assembly of bacteriophage P22 procapsids. Virology 428, 64-9. PMC3351694.
Dhason, MS, Wang, JC-Y, Hagan, M.F., Zlotnick, A (2012) Differential assembly of Hepatitis B Virus core protein on single- and double-stranded nucleic acid suggest the dsDNA-filled core is spring-loaded. Virology 430, 20-29. PMC3477361.
Wang, JC-Y, Dhason, MS, Zlotnick, A (2012) Structural organization of pregenomic RNA and the C-terminal domain in hepatitis B virus. PLoS Path 8, e1002919. PMC3447754.
Li, C, Wang, JC-Y, Taylor MW, Zlotnick, A (2012). In vitro assembly of an empty picornavirus capsid follows a dodecahedral path. J Virol. 86, 13062-9. PMC3497625.
van Eldijk, MB, Wang, JC-Y, Minten, IJ, Li, C, Zlotnick, A, Nolte, RJM, Cornelissen, JJLM, van Hest, JCM (2012) Designing Two Self-Assembly Mechanisms into One Viral Capsid Protein. JACS 134,18506-9. PMC3510441.