Mike and Yves Brun delivered a lecture to the Indiana University community entitled, “Shedding Light on the Bacterial Cell Wall.” The lecture was given as part of a symposium to recognize them as recipients of the 2014 Indiana University Outstanding Faculty Collaborative Research Award. Congratulations to Mike, Yves, and the outstanding students and postdoctoral associates that have contributed to this work.
Mike gave a lecture entitled, “Novel Chemical Probes for Use in the Study of Peptidoglycan Biosynthesis,” at the Scripps Research Institute, Jupiter, FL.
The VanNieuwenhze and Brun research groups have co-authored a manuscript entitled,”Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ.” The manuscript was published online (December 4, 2014) by Nature Protocols and will appear in print in the first issue of the journal to be published in 2015. The complete journal citation is: Kuru, E.; Tekkam, S.; Hall, E.; Brun, Y. V.*; VanNieuwenhze, M. S.,”Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ,” Nat. Protoc. 2015, 10, 33-52. DOI: 10.1038/nprot.2014.197.
The VanNieuwenhze Research Group (VRG) has collaborated with a group in France that has used our FDAA probes to investigate the organization of the peptidoglycan division machinery in Streptococcus. A manuscript detailing this work has recently (November 26, 2014) appeared online in Nature. The citation for the manuscript is: Fleurie, A.; Lesterlin, C.; Zhao, C.; Manuse, S.; Cluzel, C.; Campo, N.; Lavergne, J.-P.; Franz, M.; Macek, B.; Kuru, E.; VanNieuwenhze, M. S.; Brun, Y.; Sherratt, D.; Gangreasse, C., “Finding midcell: MapZ beacons the division sites and positions FtsZ-rings in streptococci,” Nature 2014, 516, 259-262. DOI: 10.1038/nature13966.
Jonathan Rittichier (VRG) and Erick Pasciak (Peter’s group) have collaborated of research that has culminated with a publication in the Journal of Organic Chemistry. The manuscript was published on the web on November 26, 2014. The complete citation for the manuscript is: Pasciak, E. M.; Rittichier, J. R.; Chen, C.-H.; Mubarak, M.; VanNieuwenhze, M. S.; Peters, D. G., Electroreductive Dimerization of Coumarin and Coumarin Analogues at Carbon Cathodes,” J. Org. Chem. 2014, DOI: 10.1021/jo502272g.
Garrett Booher has joined the VanNieuwenhze research group. Garrett is a first-year student in our Biochemistry Graduate Program. He completed his undergraduate study at Arizona State University and joined our BGP in the fall of 2014. Welcome Garrett!
Our work in collaboration with the the laboratory of Professor Malcolm Winkler has been highlighted with a cover photo in the October issue of Molecular Microbiology (Mol. Microbiol. 2014, 94 (1)). Click here to view the cover photo.
Srinivas Tekkam successfully presented and defended his Ph.D. thesis entitled, "Progress Towards the Total Synthesis of the Mannopeptimycins and Design and Synthesis of Dipeptide Probes for Imaging Nascent Peptidoglycan." Congratulations Srinivas! He will now move on to a postdoctoral appointment in the laboratory of Professor M. G. Finn at the Georgia Institute of Technology.
Erkin Kuru was named a recipient of a 2014 Gill Graduate Student Award by The Linda and Jack Gill Center for Biomolecular Science. Erkin will receive his award at the 2014 Gill Symposium that will be held on Wednesday, October 15, 2014 in the Wittenberger Auditorium of the Indiana Memorial Union. Congratulations Erkin!.
The VanNieuwenhze lab was awarded a grant by the National Institutes of Health to study novel inhibitors of peptidoglycan biosynthesis targeting Gram-positive pathogens.
Mike and our collaborator, Professor Yves Brun, were named winners of an inaugural Indiana University Outstanding Faculty Collaborative Research Award. Mike and Yves will deliver an award address to the university community during the Fall Semester 2014. Congratulations to all that were involved in the work to be recognized with this award!
A manuscript detailing the use of our synthetic mimics of malondialdehyde-modified LDL and their use in generating an atheroprotective response has recently appeared in the Journal of Lipid Research: Gonen, A.; Hansen, L. F.; Turner, W. W.; Montano, E. N.; Que, X.; Rafia, A.; Chou, M.-Y.; Wiesner, P.; Tsiantoulas, D.; Corr, M.; VanNieuwenhze, M. S.; Tsimikas, S.; Binder, C. J.; Witztum, J. L.; Hartvigsen, K., “Atheroprotective immunization with malondialdehyde modified LDL is hapten specific, dependent on advanced MDA-adducts: Implications for development of an atheroprotective vaccine,” J. Lipid Res., 2014, DOI: 10.1194/jlr.M053256.
A manuscript detailing the use of FDAA and b-lactam probes to study localization patterns of penicillin binding proteins has appeared in Molecular Microbiology. This was a collaborative effort with the Winkler, Brun, and Carlson laboratories:
Tsui, H.-C. T.; Boersma, M.; Vella, S.; Kocaoglu, O.; Kuru, E.; Perceny, J.; Carlson, E. E.; VanNieuwenhze, M. S.; Brun, Y. V.; Shaw, S. L.; Winkler, M. E., “Separate Localization of Penicillin-Binding Protein PBP2x from PBP2b and Other Peptidoglycan Synthesis Proteins During Later Division Stages of Streptococcus pneumonia D39,” Mol. Microbiol. 2014, DOI: 10.1111/mml.12745.
Our manuscript detailing procedures for synthesis of our “workhorse” FDAA probes, and their use in peptidoglycan labeling experiments, has been accepted by Nature Protocols: Kuru, E.; Tekkam, S.; Hall, E.; Brun, Y. V.*; VanNieuwenhze, M. S.*,”Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ.”
The Institute for Advanced Study at the University of Warwick (UK) has awarded Mike a Visiting Fellowship for the 2014-2015 academic year. The fellowship will support a sabbatical visit to Warwick during the summer of 2015.
Mike attended the 63rd Gordon Research Conference on Natural Products, July 20-25 at Proctor Academy, Andover, NH.
Erkin Kuru was selected as a recipient of a 2014 Baxter Young Investigator Award. Information about the award and the program can be found at: http://www.baxter.com/about_baxter/scientific_excellence/young_investigator/index.html. Congratulations Erkin!
Erkin attended the Metabolic Engineering X Conference (June 15-19) in Vancouver, British Columbia. While at the conference he was awarded first prize for his poster entitled, “Decorating Bacterial Surfaces by Designer Molecules Advances the Fundamental Knowledge about Bacterial Growth.” Congratulations Erkin!
Mike (“Probes for Real-Time Imaging of Peptidoglycan Synthesis in Live Bacteria”) and Erkin (A New Metabolic Labeling Method reveals Peptidoglycan in CHlamydiae”) presented posters at the 2014 Gordon Research Conference on Bacterial Cell Surfaces, June 22-27, Mount Snow Resort, West Dover, VT.
Mike gave a series of research lectures during a tour of England. He gave lectures describing our recent work with FDAAs and peptide antibiotics at The Laboratory for Molecular Biology, MRC/Cambridge; Imperial College London; The Centre for Bacterial Cell Biology, University of Newcastle; and the University of Warwick.
The University of Newcastle (UK) has named Mike a University Research Committee Visiting Fellow for the 2014-2015 academic year. This fellowship will support his sabbatical study in the Centre for Bacterial Cell Biology at the University of Newcastle.
A manuscript detailing the use of our FDAA probes for the study of proteins involved in pneumococcal cell division and elongation has appeared in print:
Fleurie, A.; Manuse, S; Zhao, C.; Campo, N.; Cluzel, C.; Lavergne, J.-P.; Freton, C.; Guiral, S.; Soufi, B.; Macek, B.; Kuru, E.; VanNieuwenhze, M. S., Brun, Y.; DiGuilmi, A.-M.; Claverys, J.-P.; Galinier, A.; Grangeasse, C., “Interplay of the serine/threonine kinase StkP and the paralogs DivIVA and GpsB in pneumococcal cell elongation and division,” PLOS Genetics 2014, DOI: 10.1371/journal.pgen.1004275.
Eddie Hall successfully defended his Ph.D. thesis entitled, “Fighting Bacterial Drug Resistance by Targeting Peptidoglycan Biosynthesis: Depsipeptide Antibiotics and Biological Probes.” He has now moved on to his postdoctoral appointment in the laboratory of Professor Karl Scheidt at Northwestern University. Congratulations Eddie!
Erkin Kuru has been named a recipient of a 2014 Walter Konetzka Fellowship. For information on Professor Konetzka and other Konetzka Fellows, please go to the following link: http://www.bio.indiana.edu/alumni/support/konetzka.shtml Congratulations, Erkin!
Erkin Kuru has been named a recipient of a 2014-2015 Dagmar K. Riley Graduate Fellowship and 2014-2015 Mabel LaDuke Lauder Fellowship. These fellowships, administered by the College of Arts and Sciences, are intended to provide support to an outstanding graduate student during their dissertation year. Congratulations, Erkin!
Eddie Hall has obtained a postdoctoral appointment in the laboratory of Professor Karl Scheidt at Northwestern University. Congratulations, Eddie!
Our Nature paper, “A new metabolic cell wall labeling method reveals peptidoglycan in Chlamydia trachomatis,” with Liechti, G.; et. al., was highlighted by the Faculty of 1000 Prime.
Michael Priestley joined the VRG. Michael is a student in the Biochemistry graduate program and he will be co-mentored by Professor Adam Zlotnick. Welcome Michael!
Yen-Pang Hsu joined the VRG. Yen-Pang is a student in the Biochemistry graduate program and he will be co-mentored by Professor Yves Brun. Welcome Yen-Pang!
Jesse Evans joined the VRG. Jesse is a student in the Biochemistry graduate program and he will be co-mentored by Professor Malcolm Winkler. Welcome Jesse!
Mike, Eddie, Erkin, former postdoctoral associate, Alvin Kalinda, and collaborator Yves Brun (Department of Biology) are coauthors on two papers, which recently appeared in Nature Communications and Nature that demonstrate, for the first time, the existence of peptidoglycan in the infamous pathogen Chlamydia, the cause of 1 in 10 cases of pneumonia in children and 212 million cases of the blindness-causing disease, trachoma. It was known that Chlamydia possessed the biosynthetic machinery to synthesize peptidoglycan and that they are sensitive to antibiotics (e.g.; the beta-lactams) that inhibit peptidoglycan biosynthesis. However, because Chlamydia is an obligate intracellular pathogen, the existence of peptidoglycan had never been unequivocally demonstrated (hence, the ‘Chlamydial anomaly’). The FDAA and dipeptide probes, developed by the VanNieuwenhze and Brun laboratories, were used to visualize the biosynthesis of peptidoglycan in Chlamydia and may provide tools for the identification of novel agents for treatment of infections due to this problematic pathogen.
A link to the IU press release: http://news.indiana.edu/releases/iu/2013/12/cell-wall-labeling-peptidoglycan.shtml
A link to the Nature Communications paper: http://www.nature.com/ncomms/2013/131202/ncomms3856/full/ncomms3856.html
A link to the Nature paper: http://www.nature.com/nature/journal/vaop/ncurrent/pdf/nature12892.pdf
This work was described in a Research Highlight, “Chlamydiae play by their own rules,” that appeared in Nature Reviews Microbiology, that was published online December 23, 2013. Link: http://www.nature.com/nrmicro/journal/vaop/ncurrent/full/nrmicro3198.html
Highlighted in, “Revealing Bacterial Building Blocks,” Inside Life Science, published online by the National Institute of General Medical Sciences, December 24, 2013. Link: http://publications.nigms.nih.gov/insidelifescience/holiday-season-cells.html
Highlighted in, “IU Researchers Solve Chlamydia Bacteria Mystery,” posted December 26, 2013. Link: http://indianapublicmedia.org/news/iu-researchers-solve-chlamydia-bacteria-mystery-60561/
Mike attended the 2013 Annual Biomedical Research Conference for Minority Students in Nashville, TN.
Ziran Li joined the VanNieuwenhze Research Group (VRG). Welcome Ziran!
Mike visited the Southern Research Institute, in Birmingham, AL, and gave a seminar entitled, “Real-Time Imaging of Peptidoglycan Synthesis in Live Bacterial Cells.”!
Mike attended the 62nd Gordon Research Conference on Natural Products at the Proctor Academy, Andover, NH.
Eddie Hall received the Eli Lilly Fellowship in Organic Chemistry. Congratulations Eddie!
Erkin was selected to attend a summer course entitled, “Physiology: Modern Cell Biology Using Microscopic, Biochemical and Computational Approaches,” at the Marine Biological Laboratory, Woods Hole Oceanographic Institute. Congratulations Erkin!
(5/3) Gu Peng successfully defended his thesis (Studies toward the Total Synthesis of Nogalamycin and Menogaril) and has accepted a postdoctoral appointment at AAT Bioquest, Inc., Sunnyvale, CA. Congratulations Gu!
(4/23) Randall Binder successfully completed his thesis defense (Studies toward the Synthesis of the Amphomycin Antibiotics: Total Synthesis of Lastpartomycin C) and has accepted a postdoctoral appointment at St. Jude Children’s Research Hospital. Congratulations Randall!
(4/26) Mike gave an invited presentation (Real-Time Imaging of Peptidoglycan Biosynthesis in Live Bacterial Cells) in the Department of Chemistry at Baylor University.
(2/20) Mike gave an invited presentation (Real-Time Imaging of Peptidoglycan Biosynthesis in Live Bacterial Cells) to the Vanderbilt University Department of Chemistry and the Vanderbilt Institute of Chemical Biology.
(10/24) Mike gave an invited talk (Bacterial Resistance, Peptide Antibiotics, and the Bacterial Cell Wall) to the University of Louisville Department of Chemistry.