Anne L. Prieto
1985 Licentiate, Biochemistry. Pontificia Universidad
Católica de Chile, Santiago, Chile.
1992 Ph.D., Biology. The Rockefeller University,
New York, NY.
Postdoctoral Studies, The Scripps Research Institute, La Jolla, CA
Email address: aprieto(at)indiana.edu
The research in my laboratory focuses on understanding the signaling mechanisms that underlie the establishment and maintenance of mature neuronal phenotypes in the developing brain. In particular, we investigate the potential functional roles of receptor protein tyrosine kinases (RPTKs) and their ligands in the central nervous system. Currently, we study a novel RPTK family, the Axl subfamily, which includes at least 4 members, Tyro-3, Axl, Mer and Rek. They share a common ligand, the molecule Gas6 (for growth-arrest specific gene-6) capable of inducing receptor phosphorylation and thus initiating intracellular signaling events upon binding. Our current efforts are focused on possible roles for these receptors and their cognate ligand in promoting the survival or growth of subsets of neural cells, in mediating cell adhesion, and in modifying synaptic function and plasticity.
Prieto, A.L., Weber, J.L, Tracy, S., Heeb, M.J, Lai, C. (1999) Gas6, a ligand for the receptor protein-tyrosine kinase Tyro-3, is widely expressed in the central nervous system. Brain Res. 816, 646-661.
Prieto, A.L., Weber, J.L, Lai, C. (2000). Expression of the receptor-protein tyrosine kinases Tyro-3, Axl and Mer in the developing rat central nervous system. J. Comp. Neurol. 425, 295-314.
Hall, M.O., Prieto, A.L., Obin, M.S, Abrams, T.A., Burgess, B.L., Heeb, M.J., Agnew, B.J. (2001). Outer segment phagocytosis by cultured retinal pigment epithelial cells requires Gas6. Exp. Eye Res. 73, 509-520.
Hall, M.O., Obin, M.S, Prieto, A.L., Burgess, B.L, Abrams, T.A. (2002). Gas6 binding to photoreceptor outer segments requires ã-carboxyglutamic acid (gla) and Ca2+ and is required for the OS phagocytosis by RPE cells in vitro. Exp. Eye Res. 75, 391-400.
Rimer, M., Prieto, A.L, Weber, J.L, Colasante, C. Ponomareva, O. Fromm, L., Schwab, M.H., Lai, C., Burden, S. (2004). Neuregulin-2 is synthesized by motor neurons and terminal Schwann cells, and activates acetylcholine receptor transcription in muscle cells expressing ErbB4. Mol. Cell. Neurosci. 26, 271-281.
Conroy, S.M., Nguyen, V., Quina, L.A., Blakely-Gonzales, P., Ur, C., Netzeband, J.G., Prieto, A.L., Gruol, D. (2004). Interleukin-6 produces neuronal loss in developing cerebellar granule neuron cultures. J. Neuroimmunol. 155, 43-54.
Prieto, A.L., O’Dell, S., Varnum, B., Lai, C (2007). Localization and signaling of the receptor protein tyrosine kinase Tyro-3 in cortical and hippocampal neurons. Neuroscience 150, 319-334.
Rimmerman, N., Hughes, H.V., Bradshaw, .H.B., Pazos, M.X., Mackie, K., Prieto, A.L., Walker, J.M.. (2008) Compartimentalization of endocannabinoids into lipid rafts in a dorsal root ganglion cell line. Brit. J of Pharma. 153, 380-389.
Tang, A., Jackson, D., Hobbs, J, Chen, W, Smith, J.L., Patel, H, Prieto, A., Petrusca, D., Grivich, M.I, Sher, A., Hottowy, P., Dabrowsky, W., Litke, A.M, Beggs, J.M. (2008) A maximum entropy model applied to spatial and temporal correlations from cortical networks in vitro. J Neurosci. 28 (2), 505-518.
Binder, M.D., Cate, H.S., Prieto, A.L., Kemper, D., Butzkueven, H., Gresle, M.M., Cipriani, T., Jokubaitis, V.G., Carmeliet, P., Kilpatrick, T.J. (2008) Gas6 deficiency increases oligodendrocyte loss and microglial activation in response to cuprizone–induced demyelination. J. Neurosci. 28, 5195-5206.
Rimmerman, N., Bradshaw, H.B., Hughes, H.V., Chen, J.S-C., Shu-Jung Hu, S,. McHugh, D., Vefring, E., Jahnsen, J.A, Thompson, E.L., Masuda, K., Cravatt, B.F., Burstein, S., Vasko, M.R., O’Dell, D.K, Prieto, A.L., Walker, J.M. (2008) N-palmitoyl glycine, a novel endogenous lipid that acts as a modulator of calcium efflux and nitric oxide production in sensory neurons. Mol. Pharmacol. 74, 213-224.
Brown, J., Pazos, M.X., Lai, C., Prieto, A.L. (2009). Signaling and proliferative functions of Tyro3 and its heterodimerization with Axl in Rat2 cells. Oncogene. In revision.