Physical Biochemistry Instrumentation Facility @ IUB

List of Equipment

*Instrument*	Make	Model	Year Purchased	Location	Major Applications
Fluorometer	Perkin Elmer	LS50B	2001	SI033	Structure, folding, binding thermodynamics and kinetics
Analytical Ultracentrifuge	Beckman Coulter	Optima XL-I	1998	SI033	Self-association (size, shape, and thermodynamics)
Isothermal Titration Calorimeter	Microcal	VP-ITC	2003	SI033	Thermodynamics of binding
Variable Mode Imaging System	Amersham Biosciences	Typhoon 9210	2003	SI033	Quantitation of gels, plates, autoradiograms, etc.
Circular Dichroism Spectrometer	Jasco	J-715	1999	A715	Structure and interactions
UV/Visible Spectrometer	Varian	Cary 100 Bio	2001	SI033	Thermodynamics of folding and kinetic assays, oligonucleotide melting
Dynamic Light Scattering	Malvern	Zetasizer Nano-S	2004	SI033	Particle size distributions, diffusion coefficients, and molecular weight information
Surface Plasmon Resonance	BIACORE	3000	2001	SI033	Kinetics of binding, binding heirarchies, concentration studies
Scintillation Counter	Packard	1600TR	1994	SI033	Quantitation of assays and substrates

Analytical Ultracentrifuge (Beckman Coulter Optima XL-I)

The analytical ultracentrifuge housed in the Facility measures solute concentration distributions in sample solutions while they are subject to high (up to 60,000 rpm) centrifugal forces. This permits characterization of thermodynamic and hydrodynamic properties of macromolecules and their interactions, such as concentration, homogeneity, sedimentation coefficient, diffusion coefficient, and buoyant density. Other parameters such as solvation, association/dissociation constants, ligand binding, and stoichiometry may also be determined. The instrument is equipped with absorbance/Rayleigh interference optics. These methods are complimentary ways to determine concentration distributions. Absorbance provides sensitive, selective solute detection. In the event that solutes do not absorb significantly (or when solvents do), refractive detection provides better results. The Beckman Coulter Optima XL-I provides a temperature range from 0° - 40° C with a selectable wavelength in the 190 – 800 nm range.