Physical Biochemistry Instrumentation Facility @ IUB

List of Equipment

*Instrument*	Make	Model	Year Purchased	Location	Major Applications
Fluorometer	Perkin Elmer	LS50B	2001	SI033	Structure, folding, binding thermodynamics and kinetics
Analytical Ultracentrifuge	Beckman Coulter	Optima XL-I	1998	SI033	Self-association (size, shape, and thermodynamics)
Isothermal Titration Calorimeter	Microcal	VP-ITC	2003	SI033	Thermodynamics of binding
Variable Mode Imaging System	Amersham Biosciences	Typhoon 9210	2003	SI033	Quantitation of gels, plates, autoradiograms, etc.
Circular Dichroism Spectrometer	Jasco	J-715	1999	A715	Structure and interactions
UV/Visible Spectrometer	Varian	Cary 100 Bio	2001	SI033	Thermodynamics of folding and kinetic assays, oligonucleotide melting
Dynamic Light Scattering	Malvern	Zetasizer Nano-S	2004	SI033	Particle size distributions, diffusion coefficients, and molecular weight information
Surface Plasmon Resonance	BIACORE	3000	2001	SI033	Kinetics of binding, binding heirarchies, concentration studies
Scintillation Counter	Packard	1600TR	1994	SI033	Quantitation of assays and substrates

Surface Plasmon Resonance (BIACORE 3000)

BIACORE permits detailed investigation of bimolecular binding without the use of conventional labels. A range of molecules from lipid vesicles, viruses, bacteria, and eukaryotic cells may be studied. The instrument provides extremely high sensitivity as well as precisely controlled experimental conditions. By immobilizing a molecule onto the sensor surface, binding events may be followed by surface plasmon resonance. As many as four separate measurements may be obtained from one chip, and automated fitting algorithms yield kinetic constants. Equipped with an autosampler, the BIACORE 3000 accommodates long, unattended runs over many hours. Sample injections may be as little as five microliters to monitor binding in all four flowcells. A broad range of sensor chip surfaces enables selection of optimal conditions for a variety of applications.