Physical Biochemistry Instrumentation Facility @ IUB

List of Equipment

*Instrument*	Make	Model	Year Purchased	Location	Major Applications
Fluorometer	Perkin Elmer	LS50B	2001	SI033	Structure, folding, binding thermodynamics and kinetics
Analytical Ultracentrifuge	Beckman Coulter	Optima XL-I	1998	SI033	Self-association (size, shape, and thermodynamics)
Isothermal Titration Calorimeter	Microcal	VP-ITC	2003	SI033	Thermodynamics of binding
Variable Mode Imaging System	Amersham Biosciences	Typhoon 9210	2003	SI033	Quantitation of gels, plates, autoradiograms, etc.
Circular Dichroism Spectrometer	Jasco	J-715	1999	A715	Structure and interactions
UV/Visible Spectrometer	Varian	Cary 100 Bio	2001	SI033	Thermodynamics of folding and kinetic assays, oligonucleotide melting
Dynamic Light Scattering	Malvern	Zetasizer Nano-S	2004	SI033	Particle size distributions, diffusion coefficients, and molecular weight information
Surface Plasmon Resonance	BIACORE	3000	2001	SI033	Kinetics of binding, binding heirarchies, concentration studies
Scintillation Counter	Packard	1600TR	1994	SI033	Quantitation of assays and substrates

Circular Dichroism Spectropolarimeter (Jasco J-715)

As linearly polarized light passes through an optically active substance, its two circularly polarized components travel at different speeds and are absorbed in differing degrees. CD is a standard method for measuring conformational changes in chiral molecules. Biochemistry researchers using the Facility are active in employing this technique for study of proteins and nucleic acids. CD spectroscopy provides information relating to the conformational arrangement molecules or assemblies and for characterizing such events as cold denaturation of RNA when coupled to thermal control of the sample. The Facility has a Jasco J-715 Circular Dichroism Spectropolarimeter interfaced with thermal sample control and SpectraManager software for instrument control and analysis.