Physical Biochemistry Instrumentation Facility @ IUB

List of Equipment

*Instrument*	Make	Model	Year Purchased	Location	Major Applications
Fluorometer	Perkin Elmer	LS50B	2001	SI033	Structure, folding, binding thermodynamics and kinetics
Analytical Ultracentrifuge	Beckman Coulter	Optima XL-I	1998	SI033	Self-association (size, shape, and thermodynamics)
Isothermal Titration Calorimeter	Microcal	VP-ITC	2003	SI033	Thermodynamics of binding
Variable Mode Imaging System	Amersham Biosciences	Typhoon 9210	2003	SI033	Quantitation of gels, plates, autoradiograms, etc.
Circular Dichroism Spectrometer	Jasco	J-715	1999	A715	Structure and interactions
UV/Visible Spectrometer	Varian	Cary 100 Bio	2001	SI033	Thermodynamics of folding and kinetic assays, oligonucleotide melting
Dynamic Light Scattering	Malvern	Zetasizer Nano-S	2004	SI033	Particle size distributions, diffusion coefficients, and molecular weight information
Surface Plasmon Resonance	BIACORE	3000	2001	SI033	Kinetics of binding, binding heirarchies, concentration studies
Scintillation Counter	Packard	1600TR	1994	SI033	Quantitation of assays and substrates

Luminescence Spectrometer (Perkin-Elmer LS50B)

The Perkin-Elmer LS50B Luminescence Spectrometer is a complete workstation for a very wide variety of sensitive biochemical studies, including fluorescence, phosphorescence, and bio-chemiluminescence. Featuring excitation and emission monochromators as well as excitation/emission polarizers suitable for fluorescence polarization, anisotropy, and ratio measurements, this instrument is routinely used for obtaining information regarding kinetics in competitive ligand binding assays on a millisecond timescale. A circulating NESLAB water bath to control sample temperature in the stirred cuvette holder is also available, as well as a scanning well plate reader. The spectrometer is equipped with FL WinLab software for completely automated instrument control, providing a platform which offers seamless data collection and analysis.