Physical Biochemistry Instrumentation Facility @ IUB

List of Equipment

*Instrument*	Make	Model	Year Purchased	Location	Major Applications
Fluorometer	Perkin Elmer	LS50B	2001	SI033	Structure, folding, binding thermodynamics and kinetics
Analytical Ultracentrifuge	Beckman Coulter	Optima XL-I	1998	SI033	Self-association (size, shape, and thermodynamics)
Isothermal Titration Calorimeter	Microcal	VP-ITC	2003	SI033	Thermodynamics of binding
Variable Mode Imaging System	Amersham Biosciences	Typhoon 9210	2003	SI033	Quantitation of gels, plates, autoradiograms, etc.
Circular Dichroism Spectrometer	Jasco	J-715	1999	A715	Structure and interactions
UV/Visible Spectrometer	Varian	Cary 100 Bio	2001	SI033	Thermodynamics of folding and kinetic assays, oligonucleotide melting
Dynamic Light Scattering	Malvern	Zetasizer Nano-S	2004	SI033	Particle size distributions, diffusion coefficients, and molecular weight information
Surface Plasmon Resonance	BIACORE	3000	2001	SI033	Kinetics of binding, binding heirarchies, concentration studies
Scintillation Counter	Packard	1600TR	1994	SI033	Quantitation of assays and substrates

Zetasizer Nano-S (Malvern Instruments)

The Malvern Instruments Zetasizer Nano-S dynamic light scattering instrument provides unequaled sensitivity and versatility in the determination of molecular weight and particle size distributions. The unique NIBS (Non-Invasive Back Scatter) technology employed allows routine measurements of particles ranging from 0.6 nm to 6 microns and molecular weight determinations from 1000 to 2 x 10⁷ Daltons, all with sample volumes as low as 12 microliters. The comprehensive analysis software calculates particle size distributions based on scattering intensity, volume, and number density as well as polydispersity. The instrument may be used to characterize sample purity, aggregations, large conformational changes, and screening for crystallization.