A wide variety of biochemical experiments require the quantitative
imaging of material (e.g. gels, plates, etc.) using phosphorescence,
chemiluminescence, or fluorescence. The Facility contains a
state-of-the-art Amersham Biosciences Typhoon 9210 instrument
complete with ImageQuant TL software for analysis and quantitation
of various samples ranging from proteins to DNA. This versatile
system accommodates sandwich, agarose, and polyacrylamide gels,
membranes, microplates, and microarrays. The high quality excitation
lasers (Red HeNe @ 633 nm or Green Nd:YAG @ 532 nm) and collection
optics allow for the sensitive detection of low abundance targets
in artifact-free images. A wide choice of emission filters enable
imaging of an extensive variety of fluorophores. Other features
include:
Automated four-color fluorescence scanning to allow multiplexing
of multiple targets in the same sample, ensuring accuracy
of analysis, increasing throughput, and saving time.
Storage phosphor technology delivers high-resolution imaging
and accurate quantitation of 3H, 14C, 32P, 33P, 35S, 125I,
and other sources of ionizing radiation.
Highly sensitive optics enable direct chemiluminescent imaging
without intermediate exposures to films or screens.
